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Literature summary for 2.3.1.47 extracted from

  • Turbeville, T.D.; Zhang, J.; Adams, W.C.; Hunter, G.A.; Ferreira, G.C.
    Functional asymmetry for the active sites of linked 5-aminolevulinate synthase and 8-amino-7-oxononanoate synthase (2011), Arch. Biochem. Biophys., 511, 107-117.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.47

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein and murine 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer) expressed in Escherichia coli HU227, R872 and DH5alpha Escherichia coli

Protein Variants

Protein Variants Comment Organism
K236A active site lysine involved in the Schiff base linkage with the PLP cofactor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 pimeloyl-CoA 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C Escherichia coli
0.025
-
 pimeloyl-CoA wild type protein, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.25
-
 L-alanine 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C Escherichia coli
0.5
-
 L-alanine wild type protein, pH not specified in the publication, temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P12998
-
-

Purification (Commentary)

Purification (Comment) Organism
from Escherichia coli DH5alpha, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, ion exchange chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + pimeloyl-CoA
-
 Escherichia coli 8-amino-7-oxononanoate + CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 x 40000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
8-amino-7-oxononanoate synthase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
 L-alanine 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C Escherichia coli
0.06
-
 L-alanine wild type protein, pH not specified in the publication, temperature not specified in the publication Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
 L-alanine 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C Escherichia coli
0.12
-
 L-alanine wild type protein, pH not specified in the publication, temperature not specified in the publication Escherichia coli
2.33
-
 pimeloyl-CoA wild type protein, pH not specified in the publication, temperature not specified in the publication Escherichia coli
2.67
-
 pimeloyl-CoA 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C Escherichia coli