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Literature summary for 2.3.1.5 extracted from

  • Walraven, J.M.; Doll, M.A.; Hein, D.W.
    Identification and characterization of functional rat arylamine N-acetyltransferase 3: comparisons with rat arylamine N-acetyltransferases 1 and 2 (2006), J. Pharmacol. Exp. Ther., 319, 369-375.
    View publication on PubMed
Search for more literature for 2.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene nat3, DNA and amino acid sequence determination and analysis, functional expression of genes nat1, nat2, and nat3 in Escherichia coli strain JM105 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0058
-
 4-aminobiphenyl recombinant isozyme Nat1 Rattus norvegicus
0.018
-
 4-aminobiphenyl recombinant isozyme Nat3 Rattus norvegicus
0.033
-
 2-Aminofluorene recombinant isozyme Nat3 Rattus norvegicus
0.037
-
 2-Aminofluorene recombinant isozyme Nat1 Rattus norvegicus
0.058
-
 N-Hydroxy-4-aminobiphenyl recombinant isozyme Nat1 Rattus norvegicus
0.062
-
 N-Hydroxy-4-aminobiphenyl recombinant isozyme Nat3 Rattus norvegicus
0.568
-
 2-Aminofluorene recombinant isozyme Nat2 Rattus norvegicus
1.57
-
 4-aminobiphenyl recombinant isozyme Nat2 Rattus norvegicus
3.3
-
 N-Hydroxy-4-aminobiphenyl recombinant isozyme Nat2 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
 x * 33500, recombinant isozyme Nat3, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an arylamine Rattus norvegicus
-
 CoA + an N-acetylarylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q80SX3 isozyme Nat3; inbred strains F344, WKY, and SPRD, isozymes Nat1, Nat2, and Nat3
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificities of isozymes Nat1-Nat3 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-aminofluorene
-
 Rattus norvegicus CoA + 2-acetylaminofluorene
-
 ?
acetyl-CoA + 3,5-dimethylaniline
-
 Rattus norvegicus CoA + N-acetyl-3,5-dimethylaniline
-
 ?
acetyl-CoA + 3-ethylaniline
-
 Rattus norvegicus CoA + N-acetyl-3-ethylaniline
-
 ?
acetyl-CoA + 4,4'-methylenebis(2-chloroaniline)
-
 Rattus norvegicus CoA + N-acetyl-4,4'-methylenebis(2-chloroaniline)
-
 ?
acetyl-CoA + 4,4'-methylenedianiline
-
 Rattus norvegicus CoA + N-acetyl-4,4'-methylenedianiline
-
 ?
acetyl-CoA + 4-aminobiphenyl
-
 Rattus norvegicus CoA + 4-acetylaminobiphenyl
-
 ?
acetyl-CoA + 5-aminosalicylic acid
-
 Rattus norvegicus CoA + 5-acetylaminosalicylic acid
-
 ?
acetyl-CoA + an arylamine
-
 Rattus norvegicus CoA + an N-acetylarylamine
-
 ?
acetyl-CoA + N-hydroxy-4-aminobiphenyl O-acetylation activity Rattus norvegicus N-hydroxy-O-acetyl-4-aminobiphenyl + CoA
-
 ?
additional information substrate specificities of isozymes Nat1-Nat3, overview Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33500, recombinant isozyme Nat3, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
NAT
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 recombinant isozyme Nat1 is completely stable for 120 min, recombinant isozyme Nat3 loses most activity within 80 min, recombinant isozyme Nat2 is almost inactivated within 5 min Rattus norvegicus