Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Bacillus cereus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1.6 M sodium citrate, pH 6.5, 0.28 M NDSB-221 as additive | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
E123D | the catalytic efficiency is 1.6-4.4 times higher than that of the wild type enzyme | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
acetyl-CoA | wild type enzyme, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C | Bacillus cereus | |
0.052 | - |
acetyl-CoA | mutant enzyme E123D, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C | Bacillus cereus | |
0.64 | - |
4-aminosalicylic acid | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
0.86 | - |
2-Aminofluorene | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
1.34 | - |
4-aminosalicylic acid | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
5.8 | - |
2-Aminofluorene | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
14.55 | - |
isoniazid | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
76.9 | - |
isoniazid | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33465 | - |
x * 33465, calculated from amino acid sequence | Bacillus cereus |
34000 | - |
x * 34000, SDS-PAGE | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Mono Q column chromatography | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-aminofluorene | - |
Bacillus cereus | CoA + N-acetyl-2-aminofluorene | - |
? | |
acetyl-CoA + 4-aminosalicylic acid | - |
Bacillus cereus | CoA + N-acetyl-4-aminosalicylate | - |
? | |
acetyl-CoA + isoniazid | - |
Bacillus cereus | CoA + N-acetylisoniazid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Bacillus cereus |
? | x * 33465, calculated from amino acid sequence | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
NAT3 | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.3 | - |
acetyl-CoA | mutant enzyme E123D, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C | Bacillus cereus | |
19.3 | - |
acetyl-CoA | wild type enzyme, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C | Bacillus cereus | |
43.9 | - |
4-aminosalicylic acid | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
44.1 | - |
2-Aminofluorene | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
56.6 | - |
4-aminosalicylic acid | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
67.4 | - |
2-Aminofluorene | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
167.1 | - |
isoniazid | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
289.3 | - |
isoniazid | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.76 | - |
isoniazid | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
11.5 | - |
isoniazid | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
11.5 | - |
2-Aminofluorene | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
42.4 | - |
4-aminosalicylic acid | wild type enzyme, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
51.3 | - |
2-Aminofluorene | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
68.4 | - |
4-aminosalicylic acid | mutant enzyme E123D, apparent value, at pH 7.5 and 25°C | Bacillus cereus | |
275 | - |
acetyl-CoA | mutant enzyme E123D, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C | Bacillus cereus | |
390 | - |
acetyl-CoA | wild type enzyme, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C | Bacillus cereus |