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Literature summary for 2.3.1.50 extracted from
- Bacterial serine palmitoyltransferase: a water-soluble homodimeric prototype of the eukaryotic enzyme (2003), Biochim. Biophys. Acta, 1647, 116-120.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | useful as a model to elucidate the reaction mechanism | Sphingomonas paucimobilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21 (DE3) pLysS, DNA sequence determination | Sphingomonas paucimobilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sphingomonas paucimobilis | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45041 | - |
2 * 45041, DNA sequence determination | Sphingomonas paucimobilis |
| 50000 | - |
2 * 50000, SDS-PAGE | Sphingomonas paucimobilis |
| 90000 | - |
gel filtration | Sphingomonas paucimobilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-serine | Sphingomonas paucimobilis | rate-limiting enzyme in synthesis of sphingolipids | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas paucimobilis | Q93UV0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant from E. coli, LCB1 and LCB2 co-immunoprecipitate | Sphingomonas paucimobilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 | formation of the external aldimine intermediate | Sphingomonas paucimobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-serine | - |
Sphingomonas paucimobilis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | rate-limiting enzyme in synthesis of sphingolipids | Sphingomonas paucimobilis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Sphingomonas paucimobilis |
| dimer | 2 * 45041, DNA sequence determination | Sphingomonas paucimobilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Sphingomonas paucimobilis | |
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Release 2023.1 (January 2023)
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