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Literature summary for 2.3.1.51 extracted from
- Topology of acyltransferase motifs and substrate specificity and accessibility in 1-acyl-sn-glycero-3-phosphate acyltransferase 1 (2007), Biochim. Biophys. Acta, 1771, 1202-1215.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Baculovirus-Sf9 insect cells, pESC-URA yeast expression vector with a c-myc-epitope, transformation into Escherichia coli after site-directed mutagenesis | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I208A | mutant of human AGPAT1 | Homo sapiens |
| P207L | mutant of human AGPAT1 | Homo sapiens |
| R149K | mutant of human AGPAT1 | Homo sapiens |
| T180S | mutant of human AGPAT1 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
palmitoyl-CoA | R149K, mutant of human AGPAT1 | Homo sapiens |  |
| 0.0036 | - |
palmitoyl-CoA | T180S, mutant of human AGPAT1 | Homo sapiens | |
| 0.004 | - |
palmitoyl-CoA | human AGPAT1 | Homo sapiens | |
| 0.0053 | - |
1-acyl-sn-glycerol 3-phosphate | I208A, mutant of human AGPAT1 | Homo sapiens | |
| 0.0054 | - |
1-acyl-sn-glycerol 3-phosphate | P207L, mutant of human AGPAT1 | Homo sapiens | |
| 0.0064 | - |
1-acyl-sn-glycerol 3-phosphate | human AGPAT1 | Homo sapiens | |
| 0.0075 | - |
palmitoyl-CoA | I208A, mutant of human AGPAT1 | Homo sapiens | |
| 0.0083 | - |
palmitoyl-CoA | P207L, mutant of human AGPAT1 | Homo sapiens | |
| 0.024 | - |
1-acyl-sn-glycerol 3-phosphate | T180S, mutant of human AGPAT1 | Homo sapiens | |
| 0.0342 | - |
1-acyl-sn-glycerol 3-phosphate | R149K, mutant of human AGPAT1 | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Homo sapiens | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q99943 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, wild-type and mutant protein | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | cDNA library | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
topology of motifs I and III confirmed by experiments with recombinant AGPAT1, catalytic roles of highly conserved residues in the four acyltransferase motifs analyzed by site-directed mutagenesis, sidechain modification of His104, Asp109, Phe146, Arg149, Glu178, Gly179, Thr180, Arg181 and Ile208 shows motif importance for catalysis, substrate accessibility to the catalytic domain analyzed by competition assay, catalytic domain not accessed by lysophosphatidic acid (LPA) with fatty acid chains shorter than 10 carbons, hydrophobicity of lysophosphatidic acid important, catalytic domain accessed by short chain acyl-CoAs but not as second substrate, motifs II and III involved in binding of lysophosphatidic acid, motifs I and IV involved in binding of acyl-CoA | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | acylation of lysophosphatidic acid to phosphatidic acid, precursor for triacylglycerol and phospholipid synthesis | Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | acyltransferase motifs I-IV analyzed by sidechain modification, substrate accessibility to the catalytic domain analyzed by competition assay | Homo sapiens | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-acyl-sn-glycero-3-phosphate acyltransferase 1 | - |
Homo sapiens |
| AGPAT1 | - |
Homo sapiens |
| lysophosphatidic acid acyltransferase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay at | Homo sapiens |
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