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Literature summary for 2.3.1.51 extracted from
- Characterization of mouse lysophosphatidic acid acyltransferase 3: an enzyme with dual functions in the testis (2009), J. Lipid Res., 50, 860-869.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| LPAAT3, phylogenetic analysis, expression of the FLAG-tagged enzyme in CHO-K1 cells | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Mus musculus | 5783 | - |
| additional information | subcellular distribution of LPAAT3, overview | Mus musculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE | Mus musculus |
| 43300 | - |
x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Mus musculus | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |  |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Mus musculus C57/BL6J | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Mus musculus | - |
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
| arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Mus musculus C57/BL6J | - |
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | Mus musculus | mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview | ? | - |
? | |
| additional information | Mus musculus C57/BL6J | mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | C4B4E7 | C57BL/6J mice | - |
| Mus musculus C57/BL6J | C4B4E7 | C57BL/6J mice | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | low expression level | Mus musculus | - |
| epididymis | low expression level | Mus musculus | - |
| heart | low expression level | Mus musculus | - |
| kidney | low expression level | Mus musculus | - |
| additional information | tissue distribution of LPAAT3 expression, overview | Mus musculus | - |
| testis | high expression level, expression of the enzyme in the testis increases significantly in an age-dependent manner | Mus musculus | - |
| TM-4 cell | testicular cell line | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity, overview | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | preferred donor substrate is arachidonoyl-CoA, no activity with oleoyl-CoA, strong activity with arachidonoyl-CoA and palmitoyl-lysophosphatidic acid, stearoyl-lysophosphatidic acid, oleoyl-lysophosphatidic acid, and octadecenyl-lysophosphatidic acid as acceptors | Mus musculus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus C57/BL6J | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | preferred donor substrate is arachidonoyl-CoA, no activity with oleoyl-CoA, strong activity with arachidonoyl-CoA and palmitoyl-lysophosphatidic acid, stearoyl-lysophosphatidic acid, oleoyl-lysophosphatidic acid, and octadecenyl-lysophosphatidic acid as acceptors | Mus musculus C57/BL6J | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
| arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus C57/BL6J | CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
| arachidonoyl-CoA + 1-octadecenyl-2-lysophosphatidylinositol | - |
Mus musculus | CoA + 1-octadecenyl-2-arachidonoylphosphatidylinositol | - |
? | |
| arachidonoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine | - |
Mus musculus | CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine | - |
? | |
| arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylinositol | - |
Mus musculus | CoA + 1-palmitoyl-2-arachidonoylphosphatidylinositol | - |
? | |
| arachidonoyl-CoA + 1-stearoyl-2-lysophosphatidic acid | - |
Mus musculus | CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | substrate specificity, overview | Mus musculus | ? | - |
? | |
| additional information | mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview | Mus musculus | ? | - |
? | |
| additional information | substrate specificity, overview | Mus musculus C57/BL6J | ? | - |
? | |
| additional information | mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview | Mus musculus C57/BL6J | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGPAT3 | - |
Mus musculus |
| LPAAT3 | - |
Mus musculus |
| lysophosphatidic acid acyltransferase 3 | - |
Mus musculus |
| More | the enzyme belongs to the 1-acylglycerol-3-phosphate O-acyltransferase, AGPAT, family | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | expression of the enzyme in the testis increases significantly in an age-dependent manner, which is regulated by induction through beta-estradiol, in TM4 cells | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme provides an an alternative important possibility to produce phosphatidylinositol in the testis | Mus musculus |
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