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Literature summary for 2.3.1.51 extracted from

  • Sukumaran, S.; Barnes, R.I.; Garg, A.; Agarwal, A.K.
    Functional characterization of the human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 10/glycerol-3-phosphate acyltransferase isoform 3 (2009), J. Mol. Endocrinol., 42, 469-478.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
AGPAT10/GPAT3, expression in CHO cells endoplasmic reticulum, and as AGPAT-active enzyme in HEK-293 cells via transfection with an adenoviral vector, overexpression of the AGPAT-active enzyme in Huh-7 cells depleted of endogenous AGPAT activity by shRNA-AGPAT2-lentivirus Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of stable Huh-7 cells with a knockout for AGPAT2 activity by shRNA-AGPAT2-lentivirus Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
oleoyl-CoA pH 7.4, 37°C, recombinant AGPAT10/GPAT3 Homo sapiens
0.0092
-
1-oleoyl-sn-glycerol 3-phosphate pH 7.4, 37°C, recombinant AGPAT10/GPAT3 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue high expression level of AGPAT10/GPAT3 and AGPAT6/GPAT4 Homo sapiens
-
brain
-
Homo sapiens
-
colon
-
Homo sapiens
-
heart
-
Homo sapiens
-
kidney
-
Homo sapiens
-
leukocyte
-
Homo sapiens
-
liver
-
Homo sapiens
-
lung high expression level of AGPAT6/GPAT4 Homo sapiens
-
additional information tissue distribution of AGPAT10/GPAT3 and AGPAT6/GPAT4 expression, overview Homo sapiens
-
ovary
-
Homo sapiens
-
pancreas very high expression level of AGPAT6/GPAT4 Homo sapiens
-
placenta
-
Homo sapiens
-
prostate gland
-
Homo sapiens
-
skeletal muscle
-
Homo sapiens
-
small intestine
-
Homo sapiens
-
spleen high expression level of AGPAT6/GPAT4 Homo sapiens
-
testis high expression level of AGPAT10/GPAT3 and AGPAT6/GPAT4 Homo sapiens
-
thymus
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of AGPAT10/GPAT3, overview Homo sapiens ?
-
?
myristoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3 Homo sapiens CoA + 1-oleoyl-2-myristoyl-sn-glycerol 3-phosphate
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 Homo sapiens CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylethanolamine about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 Homo sapiens CoA + 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylglycerol about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 Homo sapiens CoA + 1,2-dioleoyl-sn-glycero-3-phosphate
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylinositol about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 Homo sapiens CoA + 1,2-dioleoyl-sn-phosphatidylinositol
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylserine about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 Homo sapiens CoA + 1,2-dioleoyl-sn-glycerol-3-phospho-L-serine
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate oleoyl-CoA and 1-oleoyl-sn-glycerol 3-phosphate are the preferred substrates of AGPAT10/GPAT3 Homo sapiens CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
?
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3 Homo sapiens CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate
-
?
stearoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3 Homo sapiens CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
1-acylglycerol-3-phosphate-O-acyltransferase
-
Homo sapiens
1-acylglycerol-3-phosphate-O-acyltransferase isoform10/glycerol-3-phosphate acyltransferase isoform 3
-
Homo sapiens
AGPAT
-
Homo sapiens
AGPAT10/GPAT3
-
Homo sapiens
AGPAT6/GPAT4
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22
-
oleoyl-CoA pH 7.4, 37°C, recombinant AGPAT10/GPAT3 Homo sapiens