Cloned (Comment) | Organism |
---|---|
AGPAT10/GPAT3, expression in CHO cells endoplasmic reticulum, and as AGPAT-active enzyme in HEK-293 cells via transfection with an adenoviral vector, overexpression of the AGPAT-active enzyme in Huh-7 cells depleted of endogenous AGPAT activity by shRNA-AGPAT2-lentivirus | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of stable Huh-7 cells with a knockout for AGPAT2 activity by shRNA-AGPAT2-lentivirus | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
oleoyl-CoA | pH 7.4, 37°C, recombinant AGPAT10/GPAT3 | Homo sapiens | |
0.0092 | - |
1-oleoyl-sn-glycerol 3-phosphate | pH 7.4, 37°C, recombinant AGPAT10/GPAT3 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | high expression level of AGPAT10/GPAT3 and AGPAT6/GPAT4 | Homo sapiens | - |
brain | - |
Homo sapiens | - |
colon | - |
Homo sapiens | - |
heart | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
leukocyte | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
lung | high expression level of AGPAT6/GPAT4 | Homo sapiens | - |
additional information | tissue distribution of AGPAT10/GPAT3 and AGPAT6/GPAT4 expression, overview | Homo sapiens | - |
ovary | - |
Homo sapiens | - |
pancreas | very high expression level of AGPAT6/GPAT4 | Homo sapiens | - |
placenta | - |
Homo sapiens | - |
prostate gland | - |
Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
small intestine | - |
Homo sapiens | - |
spleen | high expression level of AGPAT6/GPAT4 | Homo sapiens | - |
testis | high expression level of AGPAT10/GPAT3 and AGPAT6/GPAT4 | Homo sapiens | - |
thymus | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of AGPAT10/GPAT3, overview | Homo sapiens | ? | - |
? | |
myristoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3 | Homo sapiens | CoA + 1-oleoyl-2-myristoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine | about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 | Homo sapiens | CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine | - |
? | |
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylethanolamine | about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 | Homo sapiens | CoA + 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylglycerol | about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 | Homo sapiens | CoA + 1,2-dioleoyl-sn-glycero-3-phosphate | - |
? | |
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylinositol | about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 | Homo sapiens | CoA + 1,2-dioleoyl-sn-phosphatidylinositol | - |
? | |
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylserine | about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3 | Homo sapiens | CoA + 1,2-dioleoyl-sn-glycerol-3-phospho-L-serine | - |
? | |
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | oleoyl-CoA and 1-oleoyl-sn-glycerol 3-phosphate are the preferred substrates of AGPAT10/GPAT3 | Homo sapiens | CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3 | Homo sapiens | CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3 | Homo sapiens | CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-acylglycerol-3-phosphate-O-acyltransferase | - |
Homo sapiens |
1-acylglycerol-3-phosphate-O-acyltransferase isoform10/glycerol-3-phosphate acyltransferase isoform 3 | - |
Homo sapiens |
AGPAT | - |
Homo sapiens |
AGPAT10/GPAT3 | - |
Homo sapiens |
AGPAT6/GPAT4 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
oleoyl-CoA | pH 7.4, 37°C, recombinant AGPAT10/GPAT3 | Homo sapiens |