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Literature summary for 2.3.1.54 extracted from

  • Knappe, J.; Blaschkowski, H.P.
    Pyruvate formate-lyase from Escherichia coli and its activation system (1975), Methods Enzymol., 41B, 508-518.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
oxamate obligatory component of the activation reaction Escherichia coli
pyruvate obligatory component of the activation reaction Escherichia coli
S-adenosylmethionine obligatory component of the activation reaction Escherichia coli

General Stability

General Stability Organism
virtually stable at 0°C and pH 8.0 if kept in media which display a redoxpotential of 0.2 V or below Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
CoA
-
Escherichia coli
0.051
-
acetyl-CoA
-
Escherichia coli
2
-
pyruvate
-
Escherichia coli
24
-
formate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000 Escherichia coli
140000
-
gel filtration, sedimentation velocity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA Enterococcus faecalis
-
acetyl-CoA + formate
-
r
pyruvate + CoA Clostridium sp.
-
acetyl-CoA + formate
-
r
pyruvate + CoA Escherichia coli central reaction in the anaerobic metabolism acetyl-CoA + formate
-
r

Organism

Organism UniProt Comment Textmining
Clostridium sp.
-
-
-
Enterococcus faecalis
-
previously Streptococcus faecalis
-
Escherichia coli
-
K12
-

Oxidation Stability

Oxidation Stability Organism
extreme sensitivity towards inactivation by oxygen, admittance of oxygen inactivates completely within a few seconds Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
partially Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable for at least several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + formate
-
Escherichia coli pyruvate + CoA
-
r
pyruvate + CoA
-
Escherichia coli acetyl-CoA + formate
-
r
pyruvate + CoA
-
Enterococcus faecalis acetyl-CoA + formate
-
r
pyruvate + CoA
-
Clostridium sp. acetyl-CoA + formate
-
r
pyruvate + CoA central reaction in the anaerobic metabolism Escherichia coli acetyl-CoA + formate
-
r
pyruvate + formate carboxyl group exchange reaction Escherichia coli formate + pyruvate
-
r
pyruvate + formate carboxyl group exchange reaction Enterococcus faecalis formate + pyruvate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 70000 Escherichia coli

Synonyms

Synonyms Comment Organism
PFL
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
activity declines with a half-time of 50 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.4
-
Escherichia coli