Literature summary for 2.3.1.54 extracted from

Lehtioe, L.; Leppaenen, V.M.; Kozarich, J.W.; Goldman, A.
Structure of Escherichia coli pyruvate formate-lyase with pyruvate (2002), Acta Crystallogr. Sect. D, 58, 2209-2212.

Search for more literature for 2.3.1.54

Activating Compound

Activating Compound	Comment	Organism	Structure
oxamate	obligatory component of the activation reaction	Escherichia coli
pyruvate	obligatory component of the activation reaction	Escherichia coli
S-adenosylmethionine	obligatory component of the activation reaction	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant PFL expressed in Escherichia coli BL21 (DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
space group P4(3)2(1)2, cell parameters a = b = 158.36 A, c = 159.30 A	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
85000	-	2 * 85000, homodimer, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyruvate + CoA	Escherichia coli	-	acetyl-CoA + formate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P09373	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate + CoA	-	Escherichia coli	acetyl-CoA + formate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 85000, homodimer, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
PFL	-	Escherichia coli

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Release 2023.1 (January 2023)