Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyruvate formate-lyase activating enzyme | PflA, the enzyme is required to convert pyruvate formate-lyase, PflB, from its inactive to its active, glycyl-radical-containing species | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene pflB, overexpression of the enzyme from plasmid 29 in the soluble fraction of Escherichia coli strain MC4100 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of both pflA and pflB (strain RM220) partially restores the ability of the strain to respond to exogenous formate | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the normally soluble dimeric enzyme pyruvate formate-lyase (PflB) interacts specifically with FocA, association of PflB with the cytoplasmic membrane is FocA-dependent | Escherichia coli | 5737 | - |
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + formate | Escherichia coli | - |
CoA + pyruvate | - |
? | |
additional information | Escherichia coli | pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation, molecular modeling of the FocA-PflB complex, the N-terminus of FocA is important for interaction with enzyme PflB | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09373 | gene pflB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + formate | - |
Escherichia coli | CoA + pyruvate | - |
? | |
additional information | pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation, molecular modeling of the FocA-PflB complex, the N-terminus of FocA is important for interaction with enzyme PflB | Escherichia coli | ? | - |
? | |
additional information | formate channel FocA interacts with inactive PflB in vitro, analysis by mixing equal amounts of purified Strep-tagged FocA and purified His-tagged PflB, cross-links between FocA and PflBare identified, detailed overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PflB | - |
Escherichia coli |
pyruvate formate-lyase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme pyruvate formate-lyase is responsible for intracellular formate generation in enterobacteria and other microbes | Escherichia coli |