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Literature summary for 2.3.1.54 extracted from

  • Doberenz, C.; Zorn, M.; Falke, D.; Nannemann, D.; Hunger, D.; Beyer, L.; Ihling, C.H.; Meiler, J.; Sinz, A.; Sawers, R.G.
    Pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation (2014), J. Mol. Biol., 426, 2827-2839.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
pyruvate formate-lyase activating enzyme PflA, the enzyme is required to convert pyruvate formate-lyase, PflB, from its inactive to its active, glycyl-radical-containing species Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene pflB, overexpression of the enzyme from plasmid 29 in the soluble fraction of Escherichia coli strain MC4100 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion of both pflA and pflB (strain RM220) partially restores the ability of the strain to respond to exogenous formate Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the normally soluble dimeric enzyme pyruvate formate-lyase (PflB) interacts specifically with FocA, association of PflB with the cytoplasmic membrane is FocA-dependent Escherichia coli 5737
-
membrane
-
Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + formate Escherichia coli
-
CoA + pyruvate
-
?
additional information Escherichia coli pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation, molecular modeling of the FocA-PflB complex, the N-terminus of FocA is important for interaction with enzyme PflB ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P09373 gene pflB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + formate
-
Escherichia coli CoA + pyruvate
-
?
additional information pyruvate formate-lyase interacts directly with the formate channel FocA to regulate formate translocation, molecular modeling of the FocA-PflB complex, the N-terminus of FocA is important for interaction with enzyme PflB Escherichia coli ?
-
?
additional information formate channel FocA interacts with inactive PflB in vitro, analysis by mixing equal amounts of purified Strep-tagged FocA and purified His-tagged PflB, cross-links between FocA and PflBare identified, detailed overview Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Escherichia coli

Synonyms

Synonyms Comment Organism
PflB
-
Escherichia coli
pyruvate formate-lyase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Escherichia coli

General Information

General Information Comment Organism
physiological function enzyme pyruvate formate-lyase is responsible for intracellular formate generation in enterobacteria and other microbes Escherichia coli