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Literature summary for 2.3.1.54 extracted from

  • Zhang, Y.; Dai, Z.; Krivoruchko, A.; Chen, Y.; Siewers, V.; Nielsen, J.
    Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth (2015), FEMS Yeast Res., 15, fov024 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
pyruvate formate lyase activating enzyme activates the enzyme by forming a glycine radical, cf. EC 1.97.1.4 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene pflB, coexpression of pyruvate formate lyase with the pyruvate formate lyase activating enzyme (gene pflA, UniProt ID P0A9N4) as well as with appropriate electron donors flavodoxin and ferredoxin (encoded by genes fldA and fdx, respectively), in a non-ethanol-producing Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc- MTH1-DELTAT ura3-52), plasmid maps, overview. Reduced flavodoxin is the preferred electron donor, but coexpression of either of the electron donors has a positive effect on growth under aerobic conditions. Subcloning in Escherichia coli strain DH5alpha. The PFL pathway can be functional at aerobic growth conditions in yeast when coexpressed with appropriate electron donors Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information recombinant coexpression of the enzyme with pyruvate format lyase activating enzyme and flavodoxin or ferredoxin in Saccharomyces cerevisiae leads to over 20fold increased expression of endogenous formate dehydrogenases FDH1 and FDH2 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + pyruvate Escherichia coli
-
acetyl-CoA + formate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P09373
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + pyruvate
-
Escherichia coli acetyl-CoA + formate
-
?
additional information pyruvate formate lyase (PFL) is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
PflB
-
Escherichia coli
pyruvate formate lyase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

General Information

General Information Comment Organism
physiological function pyruvate formate lyase (PFL) forms an alternative pathway for acetyl-CoA biosynthesis. Pyruvate formate lyase is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen. PFL pathway can be functional at aerobic growth conditions in yeast when coexpressed with appropriate electron donors Escherichia coli