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Literature summary for 2.3.1.8 extracted from

  • Vigenschow, H.; Schwarm, H.M.; Knobloch, K.
    Purification and characterization of a phosphotransacetylase from Rhodopseudomonas palustris (1986), Biol. Chem. Hoppe-Seyler, 367, 957-962.
    View publication on PubMed

General Stability

General Stability Organism
(NH4)2SO4, Na2SO4, NaCl or KCl stabilizes Rhodopseudomonas palustris
ammonium sulfate and potassium phosphate stabilize Rhodopseudomonas palustris
ethylene glycol 20% v/v stabilizes Rhodopseudomonas palustris
labile in dilute solutions or at elevated temperatures Rhodopseudomonas palustris
MgCl2 destabilizes Rhodopseudomonas palustris
NH4Cl sligthly stabilizes Rhodopseudomonas palustris
repeated freezing and thawing inactivates Rhodopseudomonas palustris

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 activation at low concentration, inhibition at high concentration Rhodopseudomonas palustris
coenzyme A strong substrate inhibition Rhodopseudomonas palustris
LiCl slight Rhodopseudomonas palustris
MgCl2 activation at low concentration, inhibition at high concentration Rhodopseudomonas palustris
NaCl slight Rhodopseudomonas palustris
SO42-
-
Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
CoA
-
Rhodopseudomonas palustris
4.7
-
acetyl phosphate
-
Rhodopseudomonas palustris

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Rhodopseudomonas palustris
K+ KCl activates Rhodopseudomonas palustris
NH4+ activates Rhodopseudomonas palustris
NH4+ NH4Cl activates Rhodopseudomonas palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52500
-
1 * 52500, SDS-PAGE Rhodopseudomonas palustris
54500
-
gel filtration Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + phosphate Rhodopseudomonas palustris
-
CoA + acetyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.6
-
-
Rhodopseudomonas palustris

Storage Stability

Storage Stability Organism
-24°C, (NH4)2SO4, 50-70% loss of activity after some months Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphate
-
Rhodopseudomonas palustris CoA + acetyl phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52500, SDS-PAGE Rhodopseudomonas palustris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
Rhodopseudomonas palustris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 8.0, 50 mM Tris/HCl, complete loss of activity after 1 min Rhodopseudomonas palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Rhodopseudomonas palustris