General Stability | Organism |
---|---|
(NH4)2SO4, Na2SO4, NaCl or KCl stabilizes | Rhodopseudomonas palustris |
ammonium sulfate and potassium phosphate stabilize | Rhodopseudomonas palustris |
ethylene glycol 20% v/v stabilizes | Rhodopseudomonas palustris |
labile in dilute solutions or at elevated temperatures | Rhodopseudomonas palustris |
MgCl2 destabilizes | Rhodopseudomonas palustris |
NH4Cl sligthly stabilizes | Rhodopseudomonas palustris |
repeated freezing and thawing inactivates | Rhodopseudomonas palustris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | activation at low concentration, inhibition at high concentration | Rhodopseudomonas palustris | |
coenzyme A | strong substrate inhibition | Rhodopseudomonas palustris | |
LiCl | slight | Rhodopseudomonas palustris | |
MgCl2 | activation at low concentration, inhibition at high concentration | Rhodopseudomonas palustris | |
NaCl | slight | Rhodopseudomonas palustris | |
SO42- | - |
Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
CoA | - |
Rhodopseudomonas palustris | |
4.7 | - |
acetyl phosphate | - |
Rhodopseudomonas palustris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Rhodopseudomonas palustris | |
K+ | KCl activates | Rhodopseudomonas palustris | |
NH4+ | activates | Rhodopseudomonas palustris | |
NH4+ | NH4Cl activates | Rhodopseudomonas palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
1 * 52500, SDS-PAGE | Rhodopseudomonas palustris |
54500 | - |
gel filtration | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | Rhodopseudomonas palustris | - |
CoA + acetyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodopseudomonas palustris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.6 | - |
- |
Rhodopseudomonas palustris |
Storage Stability | Organism |
---|---|
-24°C, (NH4)2SO4, 50-70% loss of activity after some months | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | - |
Rhodopseudomonas palustris | CoA + acetyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52500, SDS-PAGE | Rhodopseudomonas palustris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Rhodopseudomonas palustris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 8.0, 50 mM Tris/HCl, complete loss of activity after 1 min | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Rhodopseudomonas palustris |