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Literature summary for 2.3.1.8 extracted from

  • Rasche, M.E.; Smith, K.S.; Ferry, J.G.
    Identification of cysteine and arginine residues essential for the phosphotransacetylase from Methanosarcina thermophila (1997), J. Bacteriol., 179, 7712-7717.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
C159A Km similar to wild-type enzyme Methanosarcina thermophila
C159A/C277A/C312A/C325A Km similar to wild-type enzyme Methanosarcina thermophila
C159S Km similar to wild-type enzyme Methanosarcina thermophila
C277A Km similar to wild-type enzyme Methanosarcina thermophila
C277A/C312A/C325A Km similar to wild-type enzyme Methanosarcina thermophila
C312A Km similar to wild-type enzyme Methanosarcina thermophila
C325A Km similar to wild-type enzyme Methanosarcina thermophila
R133Q increased Km for CoA, decreased Km for acetyl phosphate Methanosarcina thermophila
R287Q decreased Km for CoA Methanosarcina thermophila
R28Q increased Km Methanosarcina thermophila
R310Q decreased Km for CoA Methanosarcina thermophila
R87Q increased Km for CoA, decreased Km for acetyl phosphate Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide
-
 Methanosarcina thermophila
Phenylglyoxal 33% inhibition after preincubation with phenylglyoxal Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 CoA R287Q mutant Methanosarcina thermophila
0.037
-
 CoA R310Q mutant Methanosarcina thermophila
0.043
-
 acetyl phosphate R87Q mutant Methanosarcina thermophila
0.071
-
 CoA C159S mutant Methanosarcina thermophila
0.073
-
 acetyl phosphate R133Q mutant Methanosarcina thermophila
0.073
-
 CoA C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
0.078
-
 CoA C325A mutant Methanosarcina thermophila
0.086
-
 CoA C277A/C312A/C325A mutant Methanosarcina thermophila
0.089
-
 CoA wild-type after expression in E. coli Methanosarcina thermophila
0.09
-
 CoA wild tpye Methanosarcina thermophila
0.092
-
 CoA C312A mutant Methanosarcina thermophila
0.093
-
 CoA C159A mutant Methanosarcina thermophila
0.093
-
 CoA C277A mutant Methanosarcina thermophila
0.166
-
 acetyl phosphate R287Q mutant Methanosarcina thermophila
0.17
-
 acetyl phosphate wild tpye Methanosarcina thermophila
0.18
-
 acetyl phosphate wild-type after expression in E. coli Methanosarcina thermophila
0.187
-
 acetyl phosphate C277A mutant Methanosarcina thermophila
0.191
-
 acetyl phosphate C159S mutant Methanosarcina thermophila
0.198
-
 acetyl phosphate C312A mutant Methanosarcina thermophila
0.206
-
 acetyl phosphate C159A mutant Methanosarcina thermophila
0.222
-
 acetyl phosphate C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
0.23
-
 acetyl phosphate C325A mutant Methanosarcina thermophila
0.253
-
 CoA R28Q mutant Methanosarcina thermophila
0.254
-
 acetyl phosphate C277A/C312A/C325A mutant Methanosarcina thermophila
0.747
-
 CoA R87Q mutant Methanosarcina thermophila
0.775
-
 acetyl phosphate R310Q mutant Methanosarcina thermophila
1.32
-
 acetyl phosphate R28Q mutant Methanosarcina thermophila
1.694
-
 CoA R133Q mutant Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + phosphate Methanosarcina thermophila
-
 CoA + acetyl phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphate
-
 Methanosarcina thermophila CoA + acetyl phosphate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.32
-
 acetyl phosphate C159A mutant Methanosarcina thermophila
1.42
-
 CoA R310Q mutant Methanosarcina thermophila
1.48
-
 CoA C159A mutant Methanosarcina thermophila
1.63
-
 acetyl phosphate R310Q mutant Methanosarcina thermophila
3.03
-
 acetyl phosphate C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
3.3
-
 CoA C159A/C277A/C312A/C325A mutant Methanosarcina thermophila
4.93
-
 acetyl phosphate R87Q mutant Methanosarcina thermophila
5.45
-
 acetyl phosphate mutant R287Q Methanosarcina thermophila
5.95
-
 CoA mutant R287Q Methanosarcina thermophila
12.5
-
 acetyl phosphate R133Q mutant Methanosarcina thermophila
24.1
-
 acetyl phosphate mutant R28Q Methanosarcina thermophila
24.6
-
 CoA R87Q mutant Methanosarcina thermophila
26
-
 CoA mutant R28Q Methanosarcina thermophila
40.6
-
 acetyl phosphate wild type Methanosarcina thermophila
44.5
-
 acetyl phosphate C312A mutant Methanosarcina thermophila
45.6
-
 CoA R133Q mutant Methanosarcina thermophila
51.6
-
 acetyl phosphate C277A/C312A/C325A mutant Methanosarcina thermophila
53.6
-
 CoA C277A/C312A/C325A mutant Methanosarcina thermophila
53.8
-
 CoA wild type Methanosarcina thermophila
55.4
-
 CoA C312A mutant Methanosarcina thermophila
57.6
-
 acetyl phosphate C325A mutant Methanosarcina thermophila
59.9
-
 CoA C325A mutant Methanosarcina thermophila
66.3
-
 acetyl phosphate C159S mutant Methanosarcina thermophila
68.5
-
 acetyl phosphate wild-type after expression in E. coli Methanosarcina thermophila
73.5
-
 CoA C159S mutant Methanosarcina thermophila
87.5
-
 CoA wild-type after expression in E. coli Methanosarcina thermophila
94.9
-
 acetyl phosphate C277A mutant Methanosarcina thermophila
100
-
 CoA C277A mutant Methanosarcina thermophila