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Literature summary for 2.3.1.84 extracted from
- Enzymatic characteristics of alcohol acetyltransferase from Hanseniaspora valbyensis, a non-Saccharomyces yeast (2008), J. Food Biochem., 32, 506-520.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Hanseniaspora valbyensis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | relative activity: 24% | Hanseniaspora valbyensis | |
| Ca2+ | relative activity: 94% | Hanseniaspora valbyensis | |
| Cd2+ | relative activity: 23% | Hanseniaspora valbyensis | |
| Cu2+ | relative activity: 20% | Hanseniaspora valbyensis | |
| EDTA | relative activity: 90% | Hanseniaspora valbyensis | |
| Hg2+ | relative activity: 3% | Hanseniaspora valbyensis | |
| Mn2+ | relative activity: 71% | Hanseniaspora valbyensis | |
| Na+ | relative activity: 98% | Hanseniaspora valbyensis | |
| Pb2+ | relative activity: 4% | Hanseniaspora valbyensis | |
| Zn2+ | relative activity: 9% | Hanseniaspora valbyensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.7 | - |
2-phenylethyl alcohol | - |
Hanseniaspora valbyensis | |
| 23.2 | - |
isoamyl alcohol | - |
Hanseniaspora valbyensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE | Hanseniaspora valbyensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hanseniaspora valbyensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme is solubilized from lipid particles using 1% Triton X-100 and then purified by three steps of chromatographic separations with diethylaminoethlyl (DEAE) Sepharose, Sephadex G-75 and Octyl Sepharose, respectively | Hanseniaspora valbyensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 2-phenylethyl alcohol | relative activity: 100% | Hanseniaspora valbyensis | CoA + 2-phenylethyl acetate | - |
? | |
| acetyl-CoA + ethanol | relative activity: 27% | Hanseniaspora valbyensis | CoA + ethyl acetate | - |
? | |
| acetyl-CoA + isoamyl alcohol | relative activity: 73% | Hanseniaspora valbyensis | CoA + isoamyl acetate | - |
? | |
| acetyl-CoA + n-butanol | relative activity: 17% | Hanseniaspora valbyensis | CoA + butyl acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAT | - |
Hanseniaspora valbyensis |
| alcohol acetyltransferase | - |
Hanseniaspora valbyensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Hanseniaspora valbyensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 30 | enzyme retains approximately 90% and 50% of the original activity after being incubated at 10°C and 30°C for 1 h, respectively | Hanseniaspora valbyensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Hanseniaspora valbyensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Hanseniaspora valbyensis |
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Release 2023.1 (January 2023)
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