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Literature summary for 2.3.1.87 extracted from

  • Ganguly, S.; Weller, J.L.; Ho, A.; Chemineau, P.; Malpaux, B.; Klein, D.C.
    Melatonin synthesis: 14-3-3-dependent activation and inhibition of arylalkylamine N-acetyltransferase mediated by phosphoserine-205 (2005), Proc. Natl. Acad. Sci. USA, 102, 1222-1227.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.87

Protein Variants

Protein Variants Comment Organism
S205A 1.6fold increase in Km-value for tryptamine compared to wild-type enzyme Ovis aries
T31A 1.7fold increase in KM-value for tryptamine compared to wild-type enzyme Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 tryptamine pH 6.8, 30°C, phosphorylated T31, unphosphorylated S205 Ovis aries
0.18
-
 tryptamine pH 6.8, 30°C, unphosphorylated enzyme Ovis aries
0.25
-
 tryptamine pH 6.8, 30°C, mutant enzyme S205A, phosphorylated T31 Ovis aries
0.28
-
 tryptamine pH 6.8, 30°C, mutant enzyme S205A, unphosphorylated Ovis aries
0.3
-
 tryptamine pH 6.8, 30°C, mutant enzyme T31A, phosphorylated S205 Ovis aries
0.31
-
 tryptamine pH 6.8, 30°C, mutant enzyme T31A, unphosphorylated Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + serotonin Ovis aries penultimate enzyme in melatonin pathway CoA + N-acetylserotonin
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein cAMP-dependent protein kinase-mediated phosphorylation of AANAT T31. Phosphorylation of T31 promotes binding of AANA to the dimeric 14-3-3 protein, which activates AANAT by increasing arylalkylamine affinity. A second AANAT cAMP-dependent protein kinase phosphorylation site, S205, is found to be 55% phosphorylated at night, when T31 is about 40% phosphorylated. AANAT is dual-phosphorylated. Light-exposure at night decreases T31 and S205 phosphorylation Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
pineal gland
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + serotonin
-
 Ovis aries CoA + N-acetylserotonin
-
 ?
acetyl-CoA + serotonin penultimate enzyme in melatonin pathway Ovis aries CoA + N-acetylserotonin
-
 ?
acetyl-CoA + tryptamine
-
 Ovis aries N-acetyltryptamine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
AANAT
-
 Ovis aries