General Stability | Organism |
---|---|
urea: 2.5 M, 17 h, 50% loss of activity, 5 M, 45 min, 50% loss of activity, 7 M, 1 min, 50% loss of activity | Zoogloea ramigera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 125fold molar excess, rapid inactivation | Zoogloea ramigera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
acetoacetyl-CoA | - |
Zoogloea ramigera | |
0.073 | - |
acetoacetyl-S-(D-pantetheine) 11-pivalate | - |
Zoogloea ramigera | |
0.074 | - |
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine | cosubstrate CoA | Zoogloea ramigera | |
0.12 | - |
acetoacetyl-S-(11-methoxymethyl)pantetheine | - |
Zoogloea ramigera | |
0.21 | - |
acetoacetyl-10-bis-demethylpantetheine 11-pivalate | - |
Zoogloea ramigera | |
0.25 | - |
acetoacetyl-S-homopantetheine 12-pivalate | - |
Zoogloea ramigera | |
0.46 | - |
acetoacetyl-S-pantetheine | - |
Zoogloea ramigera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
162000 | - |
- |
Zoogloea ramigera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | Zoogloea ramigera | involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis | acetyl-CoA + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zoogloea ramigera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sepharose, hydroxyapatite, phosphocellulose, Blue Sepharose | Zoogloea ramigera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
acetoacetyl-CoA synthesis | Zoogloea ramigera |
412 | - |
thiolysis of acetoacetyl-CoA | Zoogloea ramigera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-10-bis-demethylpantetheine 11-pivaloate | - |
Zoogloea ramigera | acetyl-CoA + acetyl-10-bis-demethylpantetheine 11-pivaloate | - |
? | |
CoA + acetoacetyl-CoA | most active in thiolysis | Zoogloea ramigera | acetyl-CoA + acetyl-CoA | - |
r | |
CoA + acetoacetyl-CoA | involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis | Zoogloea ramigera | acetyl-CoA + acetyl-CoA | - |
? | |
CoA + acetoacetyl-S-(11-methoxymethyl)pantetheine | - |
Zoogloea ramigera | acetyl-CoA + acetyl-S-(11-methoxymethyl)pantetheine | - |
? | |
CoA + acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine | - |
Zoogloea ramigera | acetyl-CoA + acetyl-S-(11-t-butyldimethylsilyl)pantetheine | - |
? | |
CoA + acetoacetyl-S-(D-pantetheine) 11-pivalate | - |
Zoogloea ramigera | acetyl-CoA + acetyl-S-(D-pantetheine) 11-pivalate | - |
? | |
CoA + acetoacetyl-S-(L-pantetheine) 11-pivalate | - |
Zoogloea ramigera | acetyl-CoA + acetyl-S-(L-pantetheine) 11-pivalate | - |
? | |
CoA + acetoacetyl-S-homopantetheine 12-pivalate | - |
Zoogloea ramigera | acetyl-CoA + acetyl-S-homopantetheine 12-pivalate | - |
? | |
CoA + acetoacetyl-S-pantetheine | - |
Zoogloea ramigera | acetyl-CoA + acetyl-S-pantetheine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
174 | - |
acetoacetyl-S-pantetheine | - |
Zoogloea ramigera | |
177 | - |
acetoacetyl-S-homopantetheine 12-pivalate | - |
Zoogloea ramigera | |
256 | - |
acetoacetyl-S-(L-pantetheine) 11-pivalate | - |
Zoogloea ramigera | |
266 | - |
acetoacetyl-10-bis-demethylpantetheine 11-pivaloate | - |
Zoogloea ramigera | |
353 | - |
acetoacetyl-S-(11-methoxymethyl)pantetheine | - |
Zoogloea ramigera | |
434 | - |
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine | - |
Zoogloea ramigera | |
465 | - |
acetoacetyl-CoA | - |
Zoogloea ramigera | |
469 | - |
acetoacetyl-S-(D-pantetheine) 11-pivalate | - |
Zoogloea ramigera |