Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.9 extracted from

  • Wiesenborn, D.P.; Rudolph, F.B.; Papoutsakis, E.T.
    Thiolase from Clostridium acetobutylicum ATCC 824 and its role in the synthesis of acids and solvents (1988), Appl. Environ. Microbiol., 54, 2717-2722.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
ATP 10 mM, 41% inhibition Clostridium acetobutylicum
butyryl-CoA 1 mM, 42% inhibition Clostridium acetobutylicum
CoA inhibition in condensation direction Clostridium acetobutylicum
Dithionitrobenzoate low but significant inhibition Clostridium acetobutylicum
iodoacetamide 0.3 mM, 93% inhibition after 30 min Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
CoA
-
Clostridium acetobutylicum
0.032
-
acetoacetyl-CoA
-
Clostridium acetobutylicum
0.27
-
acetyl-CoA
-
Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
4 * 44000, SDS-PAGE Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Clostridium acetobutylicum
-
acetyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
ATCC 824
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephacel, Blue Sepharose, AX 300 column Clostridium acetobutylicum

Reaction

Reaction Comment Organism Reaction ID
2 acetyl-CoA = CoA + acetoacetyl-CoA mechanism Clostridium acetobutylicum
2 acetyl-CoA = CoA + acetoacetyl-CoA ping-pong mechanism Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
216
-
-
Clostridium acetobutylicum

Storage Stability

Storage Stability Organism
4°C, 1 mM dithiothreitol, 10% v/v glycerol, N2 atmosphere, 1 month, less than 10% loss of activity Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
Clostridium acetobutylicum acetyl-CoA + acetyl-CoA
-
?
CoA + acetoacetyl-CoA
-
Clostridium acetobutylicum acetyl-CoA + acetyl-CoA
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.9
-
Clostridium acetobutylicum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 69% of maximal activity at pH 9.0 Clostridium acetobutylicum