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Literature summary for 2.3.1.9 extracted from

  • Hedl, M.; Sutherlin, A.; Wilding, E.I.; Mazzulla, M.; McDevitt, D.; Lane, P.; Burgner, J.W.; Lehnbeuter, K.R.; Stauffacher, C.V.; Gwynn, M.N.; Rodwell, V.W.
    Enterococcus faecalis acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, a dual-function protein of isopentenyl diphosphate biosynthesis (2002), J. Bacteriol., 184, 2116-2122.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
CoA
-
Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
CoA thiolysis Enterococcus faecalis
0.088
-
acetoacetyl-CoA thiolysis Enterococcus faecalis
0.6
-
acetyl-CoA synthesis Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86496
-
x * 86496, deduced from nucleotide sequence Enterococcus faecalis
88712
-
x * 88712, MALDI-mass spectrometry Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Enterococcus faecalis
-
acetyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged thiolase, Ni-affinity column Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
Enterococcus faecalis acetyl-CoA + acetyl-CoA
-
?
CoA + acetoacetyl-CoA enzyme has both thiolase and 3-hydroxy-3-methylglutaryl-CoA reductase activity Enterococcus faecalis acetyl-CoA + acetyl-CoA
-
r

Subunits

Subunits Comment Organism
? x * 88712, MALDI-mass spectrometry Enterococcus faecalis
? x * 86496, deduced from nucleotide sequence Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
acetoacetyl-CoA synthesis and thiolysis Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
thiolysis Enterococcus faecalis
10.5
-
synthesis Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.12
-
CoA
-
Enterococcus faecalis