KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
putrescine | retinoic-acid-induced enzyme | Mus musculus | |
0.065 | - |
putrescine | retinoic acid induced transglutaminase | Mus musculus | |
0.098 | 0.106 | putrescine | enzyme induced by 12-O-tetradecanoylphorbol-13-acetate or Ca2+ | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | retinoic acid induced enzyme | Mus musculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | Mus musculus | epidermal enzyme involved in formation of cornified envelope | protein N5-alkylglutamine + NH3 | - |
? | |
protein-bound gamma-glutamine + alkylamine | Mus musculus BALB/c | epidermal enzyme involved in formation of cornified envelope | protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
BALB/c strain | - |
Mus musculus BALB/c | - |
BALB/c strain | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal cell | primary and MCA3A1-cell line, retinoic acid induced enzyme differs from normal epidermal enzyme | Mus musculus | - |
epidermis | subcellular distribution | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | epidermal enzyme involved in formation of cornified envelope | Mus musculus | protein N5-alkylglutamine + NH3 | - |
? | |
protein-bound gamma-glutamine + alkylamine | epidermal enzyme involved in formation of cornified envelope | Mus musculus BALB/c | protein N5-alkylglutamine + NH3 | - |
? | |
putrescine + casein | - |
Mus musculus | ? | - |
? | |
putrescine + casein | - |
Mus musculus BALB/c | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
at least 30 min stable, pH 9.0, retinoic acid induced enzyme | Mus musculus |
37 | - |
retinoic acid induced transgutaminase, loss of approx. 75% activity after 10 min at pH 9, epidermal transglutaminase, 25% loss of activity after 20 min, pH 9.0 | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Mus musculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
thermolabile: t1/2: 3-4 min, 37°C, retinoic acid induced enzyme, 20% loss of activity within 20 min, epidermal enzyme | Mus musculus |