Crystallization (Comment) | Organism |
---|---|
docking model of MTG binding to substrate N-carbobenzoxy-L-glutaminyl-glycine. Substrate is stretched along the MTG active site cleft with hydrophobic and/or aromatic residues interacting directly with the substrate. An oxyanion binding site for TGase activity may be constructed from the amide groups of Cys64 and/or Val65 | Streptomyces mobaraensis |
Protein Variants | Comment | Organism |
---|---|---|
C64A | complete loss of activity | Streptomyces mobaraensis |
C64A | 0.4% residual activity | Streptomyces mobaraensis |
D255A | complete loss of activity | Streptomyces mobaraensis |
D255A | 0.2% residual activity | Streptomyces mobaraensis |
D304A | 41.1% residual activity | Streptomyces mobaraensis |
E300A | 54.7% residual activity | Streptomyces mobaraensis |
F254A | complete loss of activity | Streptomyces mobaraensis |
F305A | 18.6% residual activity | Streptomyces mobaraensis |
G63A | complete loss of activity | Streptomyces mobaraensis |
H274A | 9.3% residual activity | Streptomyces mobaraensis |
H277A | complete loss of activity | Streptomyces mobaraensis |
I240A | 68.3% residual activity | Streptomyces mobaraensis |
L285A | 36.8% residual activity | Streptomyces mobaraensis |
N253A | complete loss of activity | Streptomyces mobaraensis |
N253A | 1.1% residual activity | Streptomyces mobaraensis |
N276A | 1.7% residual activity | Streptomyces mobaraensis |
R26A | 18% residual activity | Streptomyces mobaraensis |
V252A | 6.0% residual activity | Streptomyces mobaraensis |
V65A | 10.2% residual activity | Streptomyces mobaraensis |
Y256A | complete loss of activity | Streptomyces mobaraensis |
Y256A | 1.9% residual activity | Streptomyces mobaraensis |
Y278A | 3.9% residual activity | Streptomyces mobaraensis |
Y62A | complete loss of activity | Streptomyces mobaraensis |
Y75A | 5.3% residual activity | Streptomyces mobaraensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | P81453 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbobenzoxy-L-glutaminyl-glycine + ovalbumin | - |
Streptomyces mobaraensis | ? | - |
? |