Literature summary for 2.3.2.13 extracted from

Wang, Z.; Collighan, R.J.; Pytel, K.; Rathbone, D.L.; Li, X.; Griffin, M.
Characterization of heparin-binding site of tissue transglutaminase: its importance in cell surface targeting, matrix deposition, and cell signaling (2012), J. Biol. Chem., 287, 13063-13083.

Search for more literature for 2.3.2.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in HEK-293T/17, NIH-3T3, CHO-K1, and opossum kidney cells	Cavia porcellus

Protein Variants

Protein Variants	Comment	Organism
C277S	the mutant binds to heparin with about wild type affinity	Cavia porcellus
D94A/D97A	the mutant is found at comparable levels on the cell surface with that shown for the wild type enzyme	Cavia porcellus
K205A/R209A	the mutant shows increased activity in NIH 3T3 and HEK-293/T17 cell lysates compared to the wild type enzyme	Cavia porcellus
K60A/R601A/K602A	the mutant shows reduced activity in NIH 3T3 cell lysates and increased activity in HEK-293/T17 cell lysates compared to the wild type enzyme	Cavia porcellus
additional information	the TG2DELTA1-15 mutant is not detectable within the extracellular matrix	Cavia porcellus

Inhibitors

Inhibitors	Comment	Organism	Structure
R281	irreversible inhibitor	Cavia porcellus
R283	irreversible inhibitor	Cavia porcellus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	heparin sulfate binding is required for extracellular matrix localization of the enzyme	Cavia porcellus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	dependent on	Cavia porcellus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
fibronectin-bound gamma-glutamine + alkylamine	Cavia porcellus	-	fibronectin N5-alkylglutamine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Cavia porcellus	Q7M0F8	-	-

Purification (Commentary)

Purification (Comment)	Organism
heparin Sepharose column chromatography	Cavia porcellus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Cavia porcellus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
fibronectin-bound gamma-glutamine + alkylamine	-	Cavia porcellus	fibronectin N5-alkylglutamine + NH3	-	?

Synonyms

Synonyms	Comment	Organism
TG2	-	Cavia porcellus
tissue transglutaminase	-	Cavia porcellus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)