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Literature summary for 2.3.2.13 extracted from
- Expression, purification and kinetic characterisation of human tissue transglutaminase (2013), Protein Expr. Purif., 87, 41-46.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | 48% residual activity at 5% (v/v) | Homo sapiens |  |
| acrylonitrile | 68 and 48% residual activity at 2.5 and 5.0 mM, respectively | Homo sapiens | |
| CP30a | reversible inhibitor | Homo sapiens | |
| CP4d | reversible inhibitor | Homo sapiens | |
| DMF | 90% residual activity at 2.5 and 5% (v/v) | Homo sapiens | |
| NC-I052 | irreversible inhibitor | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
N-acetyl-PNPQLPF | apparent value, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C | Homo sapiens | |
| 0.014 | - |
Ac-PNPQLPF-OH | at pH 7.0 and 25°C | Homo sapiens | |
| 0.014 | - |
N-Cbz-L-glutaminyl(gamma-4-nitrophenylester)glycine | 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77600 | - |
x * 77600, untagged enzyme, estimated from SDS-PAGE | Homo sapiens |
| 77600 | - |
x * 77600, calculated from SDS-PAGE | Homo sapiens |
| 104000 | - |
x * 104000, GST and PreScission Protease-tagged enzyme, estimated from SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-PNPQLPF + alkylamine | Homo sapiens | - |
? | - |
? | |
| N-Cbz-L-glutaminyl(gamma-4-nitrophenylester)glycine + alkylamine | Homo sapiens | - |
? | - |
? | |
| [protein]-L-glutamine + alkylamine | Homo sapiens | - |
[protein]-N5-alkyl-L-glutamine + NH3 | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dimethylformamide | about 90% activity at 2.5 and 5.0 mM dimethylformamide | Homo sapiens |
| DMSO | 100% activity in the presence of 2.5 and 5.0 mM DMSO | Homo sapiens |
| additional information | the enzyme is not affected by DMSO | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P21980 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione Sepharose 4B resin chromatography | Homo sapiens |
| glutathione Sepharose column chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
recombinant enzyme from lysate supernatant, at pH 7.0 and 25°C | Homo sapiens |
| 0.01 | - |
unpurified enzyme, with N-Cbz-Glu(gamma-4-nitrophenylester)Gly as substrate, at pH 7.0 and 25°C | Homo sapiens |
| 0.33 | - |
purified recombinant enzyme, at pH 7.0 and 25°C | Homo sapiens |
| 0.33 | - |
purified enzyme, with N-Cbz-Glu(gamma-4-nitrophenylester)Gly as substrate, at pH 7.0 and 25°C | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, several months, no loss of activity | Homo sapiens |
| 4°C, lyophilised purified enzyme, 1 week, 80% loss of activity | Homo sapiens |
| 4°C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, 1 week, 10% loss of activity | Homo sapiens |
| 4°C, purified enzyme, 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0, 2 weeks, 25% loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ac-PNPQLPF-OH + alkylamine | - |
Homo sapiens | ? | - |
? | |
| N-acetyl-PNPQLPF + alkylamine | - |
Homo sapiens | ? | - |
? | |
| N-Cbz-Glu(gamma-4-nitrophenylester)Gly + alkylamine | - |
Homo sapiens | 4-nitrophenol + ? | - |
? | |
| N-Cbz-L-glutaminyl(gamma-4-nitrophenylester)glycine + alkylamine | - |
Homo sapiens | ? | - |
? | |
| [protein]-L-glutamine + alkylamine | - |
Homo sapiens | [protein]-N5-alkyl-L-glutamine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 104000, GST and PreScission Protease-tagged enzyme, estimated from SDS-PAGE | Homo sapiens |
| ? | x * 77600, untagged enzyme, estimated from SDS-PAGE | Homo sapiens |
| ? | x * 77600, calculated from SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TG2 | - |
Homo sapiens |
| TGase | - |
Homo sapiens |
| tissue transglutaminase | - |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
CP4d | at pH 7.0 and 25°C | Homo sapiens | |
| 0.001 | - |
CP4d | 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C | Homo sapiens | |
| 0.004 | - |
CP30a | at pH 7.0 and 25°C | Homo sapiens | |
| 0.004 | - |
CP30a | 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C | Homo sapiens | |
| 0.019 | - |
NC-I052 | at pH 7.0 and 25°C | Homo sapiens | |
| 0.019 | - |
NC-I052 | 0.05 mM EDTA, 3.3 mM CaCl2, in 0.11 M MOPS at pH 7.0 and 25°C | Homo sapiens | |
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