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Literature summary for 2.3.2.13 extracted from

  • Liu, S.; Wan, D.; Wang, M.; Madzak, C.; Du, G.; Chen, J.
    Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization (2015), BMC Biotechnol., 15, 75.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.13

Cloned(Commentary)

Cloned (Comment) Organism
the pro-enzyme is expressed in Yarrowia lipolytica Po1g or Po1h strains Streptomyces hygroscopicus

Protein Variants

Protein Variants Comment Organism
N160Q the mutant shows wild type activity Streptomyces hygroscopicus
N160Q/N355Q the mutant shows 57% activity compared to the wild type enzyme Streptomyces hygroscopicus
N355Q the mutant shows 147% activity compared to the wild type enzyme Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
47
-
 N-Cbz-Gln-Gly mutant enzyme N160Q, at pH 6.0 and 37°C Streptomyces hygroscopicus
48.4
-
 N-Cbz-Gln-Gly mutant enzyme N160Q/N355Q, at pH 6.0 and 37°C Streptomyces hygroscopicus
54.7
-
 N-Cbz-Gln-Gly mutant enzyme N355Q, at pH 6.0 and 37°C Streptomyces hygroscopicus
59.5
-
 N-Cbz-Gln-Gly wild type enzyme, at pH 6.0 and 37°C Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43600
-
 x * 43600, pro-enzyme, calculated from amino acid sequence Streptomyces hygroscopicus
45000
-
 x * 45000, pro-enzyme, SDS-PAGE Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-L-glutamine + alkylamine Streptomyces hygroscopicus
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?
[protein]-L-glutamine + alkylamine Streptomyces hygroscopicus WSH03-13
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus D9J009
-
-
Streptomyces hygroscopicus WSH03-13 D9J009
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Streptomyces hygroscopicus

Purification (Commentary)

Purification (Comment) Organism
Fractogel EMD SO3- column chromatography Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Cbz-Gln-Gly + alkylamine
-
 Streptomyces hygroscopicus ? + NH3
-
 ?
N-Cbz-Gln-Gly + alkylamine
-
 Streptomyces hygroscopicus WSH03-13 ? + NH3
-
 ?
[protein]-L-glutamine + alkylamine
-
 Streptomyces hygroscopicus [protein]-N5-alkyl-L-glutamine + NH3
-
 ?
[protein]-L-glutamine + alkylamine
-
 Streptomyces hygroscopicus WSH03-13 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 43600, pro-enzyme, calculated from amino acid sequence Streptomyces hygroscopicus
? x * 45000, pro-enzyme, SDS-PAGE Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
TGase
-
 Streptomyces hygroscopicus
transglutaminase
-
 Streptomyces hygroscopicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Streptomyces hygroscopicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 50 the half-life of the recombinant wild type pro-enzyme at 37°C reaches 34 min. The zymogen retains approximately 80% of the activity after incubation at 20°C for 30 min and is approximately inactivated after the same time at 50°C Streptomyces hygroscopicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.6
-
 N-Cbz-Gln-Gly mutant enzyme N160Q/N355Q, at pH 6.0 and 37°C Streptomyces hygroscopicus
23.8
-
 N-Cbz-Gln-Gly mutant enzyme N355Q, at pH 6.0 and 37°C Streptomyces hygroscopicus
25.3
-
 N-Cbz-Gln-Gly mutant enzyme N160Q, at pH 6.0 and 37°C Streptomyces hygroscopicus
31.5
-
 N-Cbz-Gln-Gly wild type enzyme, at pH 6.0 and 37°C Streptomyces hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Streptomyces hygroscopicus