Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 20 mM MES at pH 6.8, 200 mM sodium chloride, 20 mM MgCl2, 6% (w/v) PEG 3350, 5 mM dithiothreitol, and 24% (v/v) glycerol | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78520 | - |
1 * 78520, including the C-terminal His-tag and GTP, calculated from amino acid sequence | Homo sapiens |
78570 | - |
1 * 78570, multiangle light scattering | Homo sapiens |
80000 | - |
1 * 80000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-L-glutamine + alkylamine | Homo sapiens | - |
[protein]-N5-alkyl-L-glutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity resin column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-L-glutamine + alkylamine | - |
Homo sapiens | [protein]-N5-alkyl-L-glutamine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Homo sapiens |
monomer | 1 * 78520, including the C-terminal His-tag and GTP, calculated from amino acid sequence | Homo sapiens |
monomer | 1 * 78570, multiangle light scattering | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TG2 | - |
Homo sapiens |
transglutaminase2 | - |
Homo sapiens |