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Literature summary for 2.3.2.13 extracted from
- Expression of Zea mays transglutaminase in Pichia pastoris under different promoters and its impact on properties of acidified milk protein concentrate gel (2019), J. Sci. Food Agric., 99, 4518-4523 .
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | enzyme TGZ treatment effectively improves the textural properties of milk protein concentrate (MPC) gel at strength level and water-holding capacity. Optimal texture of MPC gel is achieved after TGZ treatment using 2 U/g TGZ for 2 h at 35°C and pH 7.0, method evaluation and optimization, overview | Zea mays |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tgz, recombinant expression of wild-type and codon-optimized enzyme in Pichia pastoris strain GS115 under control of two different promoter types, PFLD1 and PTEF1. Higher expression of TGZ is obtained under the induction of PFLD1 | Zea mays |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Zea mays |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein glutamine + alkylamine | Zea mays | - |
protein N5-alkylglutamine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant expression of wild-type and codon-optimized, engineered enzymes from Pichia pastoris strain GS115 by ultrafiltration, gel filtration, anion exchange chromatography, and a second step of gel filtration | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.32 | - |
recombinant wild-type enzyme, about, pH 7.0, 30°C | Zea mays |
| 0.889 | - |
recombinant engineered and optimized enzyme, pH 7.0, 30°C | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein glutamine + hydroxylamine | - |
Zea mays | casein N5-hydroxyglutamine + NH3 | - |
? | |
| N-CBZ-Glu-Gly + hydroxylamine | - |
Zea mays | CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Zea mays | protein N5-alkylglutamine + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TGase | - |
Zea mays |
| TGZ | - |
Zea mays |
| transglutaminase | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Zea mays |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | transglutaminase (TGase) catalyzes post-translational modification of proteins by gamma-glutamyl-epsilon-lysine chain links, covalent conjugation of polyamines, and deamidation. Analysis of cross-linking effect of recombinant TGZ on the properties of acid-induced milk protein concentrate (MPC) gel | Zea mays |
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Release 2023.1 (January 2023)
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