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Literature summary for 2.3.2.15 extracted from

  • Beck, A.; Lendzian, K.; Oven, M.; Christmann, A.; Grill, E.
    Phytochelatin synthase catalyzes key step in turnover of glutathione conjugates (2003), Phytochemistry, 62, 423-431.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
bimane-glutathione
-
Silene vulgaris
7.5
-
glutathione
-
Silene vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activation Arabidopsis thaliana
Cd2+ activation Silene vulgaris
Cd2+ strongly dependent, optimal concentration 0.03 mM Arabidopsis thaliana
Cu2+ activation Arabidopsis thaliana
Cu2+ activation Silene vulgaris
Cu2+ optimal at 0.04 mM, can replace Cd2+ Arabidopsis thaliana
Zn2+ activation Arabidopsis thaliana
Zn2+ activation Silene vulgaris
Zn2+ optimal at 0.1 mM, can replace Cd2+ Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly Silene vulgaris
-
Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana n = 2-11 Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana Heyn. n = 2-11 Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana Heyn. cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly Silene vulgaris Wib.
-
Gly + [Glu(-Cys)]n+1-Gly
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Heyn.
-
-
-
Silene vulgaris
-
-
-
Silene vulgaris Wib.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Silene vulgaris

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HCl, pH 8.0, 1 mM mercaptoethanol, 20% glycerol and 1% bovine serum albumin Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamido-fluorescein-glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n-Glu-S-acetamido-fluorescein-Cys-Gly
-
?
benzyl-glutathione + [Glu(-Cys)]n Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n-Glu-S-benzyl-Cys-Gly
-
?
bimane-glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly
-
?
bimane-glutathione + [Glu(-Cys)]n-Gly
-
Silene vulgaris Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly
-
?
bimane-glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Heyn. Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly
-
?
bimane-glutathione + [Glu(-Cys)]n-Gly
-
Silene vulgaris Wib. Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly
-
Silene vulgaris Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly n = 2-11 ?
glutathione + [Glu(-Cys)]n-Gly n = 2-11 Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Heyn. Gly + [Glu(-Cys)]n+1-Gly n = 2-11 ?
glutathione + [Glu(-Cys)]n-Gly n = 2-11 Arabidopsis thaliana Heyn. Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates Arabidopsis thaliana Heyn. Gly + [Glu(-Cys)]n+1-Gly
-
?
glutathione + [Glu(-Cys)]n-Gly
-
Silene vulgaris Wib. Gly + [Glu(-Cys)]n+1-Gly
-
?
nitrobenzyl-glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n-Glu-S-nitrobenzyl-Cys-Gly
-
?
uracil-glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Gly + [Glu(-Cys)]n-Glu-S-uracil-Cys-Gly
-
?
uracil-glutathione + [Glu(-Cys)]n-Gly
-
Arabidopsis thaliana Heyn. Gly + [Glu(-Cys)]n-Glu-S-uracil-Cys-Gly
-
?

Synonyms

Synonyms Comment Organism
PCS
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9 8.5
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Arabidopsis thaliana