Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
bimane-glutathione | - |
Silene vulgaris | |
7.5 | - |
glutathione | - |
Silene vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activation | Arabidopsis thaliana | |
Cd2+ | activation | Silene vulgaris | |
Cd2+ | strongly dependent, optimal concentration 0.03 mM | Arabidopsis thaliana | |
Cu2+ | activation | Arabidopsis thaliana | |
Cu2+ | activation | Silene vulgaris | |
Cu2+ | optimal at 0.04 mM, can replace Cd2+ | Arabidopsis thaliana | |
Zn2+ | activation | Arabidopsis thaliana | |
Zn2+ | activation | Silene vulgaris | |
Zn2+ | optimal at 0.1 mM, can replace Cd2+ | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | Silene vulgaris | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | n = 2-11 | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana Heyn. | n = 2-11 | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana Heyn. | cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | Silene vulgaris Wib. | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana Heyn. | - |
- |
- |
Silene vulgaris | - |
- |
- |
Silene vulgaris Wib. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Silene vulgaris |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Tris-HCl, pH 8.0, 1 mM mercaptoethanol, 20% glycerol and 1% bovine serum albumin | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetamido-fluorescein-glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n-Glu-S-acetamido-fluorescein-Cys-Gly | - |
? | |
benzyl-glutathione + [Glu(-Cys)]n Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n-Glu-S-benzyl-Cys-Gly | - |
? | |
bimane-glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly | - |
? | |
bimane-glutathione + [Glu(-Cys)]n-Gly | - |
Silene vulgaris | Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly | - |
? | |
bimane-glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana Heyn. | Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly | - |
? | |
bimane-glutathione + [Glu(-Cys)]n-Gly | - |
Silene vulgaris Wib. | Gly + [Glu(-Cys)]n-Glu-S-bimane-Cys-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | - |
Silene vulgaris | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | n = 2-11 | ? | |
glutathione + [Glu(-Cys)]n-Gly | n = 2-11 | Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates | Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana Heyn. | Gly + [Glu(-Cys)]n+1-Gly | n = 2-11 | ? | |
glutathione + [Glu(-Cys)]n-Gly | n = 2-11 | Arabidopsis thaliana Heyn. | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | cellular functions are formation of heavy-metal binding peptides and degradation of glutathione-S-conjugates | Arabidopsis thaliana Heyn. | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
glutathione + [Glu(-Cys)]n-Gly | - |
Silene vulgaris Wib. | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
nitrobenzyl-glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n-Glu-S-nitrobenzyl-Cys-Gly | - |
? | |
uracil-glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n-Glu-S-uracil-Cys-Gly | - |
? | |
uracil-glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana Heyn. | Gly + [Glu(-Cys)]n-Glu-S-uracil-Cys-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PCS | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.5 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Arabidopsis thaliana |