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Literature summary for 2.3.2.15 extracted from

  • Grill, E.; Löffler, S.; Winnacker, E.L.; Zenk, M.H.
    Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase) (1989), Proc. Natl. Acad. Sci. USA, 86, 6838-6842.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 monobromobimane-glutathione
-
 Silene vulgaris
6.7
-
 glutathione
-
 Silene vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ activation Silene vulgaris
Bi3+ activation Silene vulgaris
Cd2+ activation Silene vulgaris
Cu2+ activation Silene vulgaris
Hg2+ activation Silene vulgaris
Pb2+ activation Silene vulgaris
Zn2+ activation Silene vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 4 * 25000, SDS-PAGE, dimer also catalytically active Silene vulgaris
95000
-
 gel filtration Silene vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly Podophyllum peltatum n = 2-5 Gly + [Glu(-Cys)]n+1-Gly
-
 ?
glutathione + [Glu(-Cys)]n-Gly Silene vulgaris critical for heavy metal tolerance Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
-
-
Equisetum giganteum
-
-
-
Eschscholzia californica
-
-
-
Podophyllum peltatum
-
-
-
Silene vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Silene vulgaris

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol, 0.1% NaN3 Silene vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
 Beta vulgaris Gly + [Glu(-Cys)]n+1-Gly
-
 ?
glutathione + [Glu(-Cys)]n-Gly
-
 Eschscholzia californica Gly + [Glu(-Cys)]n+1-Gly
-
 ?
glutathione + [Glu(-Cys)]n-Gly
-
 Silene vulgaris Gly + [Glu(-Cys)]n+1-Gly
-
 ?
glutathione + [Glu(-Cys)]n-Gly
-
 Equisetum giganteum Gly + [Glu(-Cys)]n+1-Gly
-
 ?
glutathione + [Glu(-Cys)]n-Gly
-
 Podophyllum peltatum Gly + [Glu(-Cys)]n+1-Gly n = 2-5 ?
glutathione + [Glu(-Cys)]n-Gly n = 2-5 Podophyllum peltatum Gly + [Glu(-Cys)]n+1-Gly
-
 ?
glutathione + [Glu(-Cys)]n-Gly critical for heavy metal tolerance Silene vulgaris Gly + [Glu(-Cys)]n+1-Gly
-
 ?
monobromobimane-glutathione + [Glu(-Cys)]n-Gly
-
 Silene vulgaris Gly + [Glu(-Cys)]n-Glu-S-monobromobimane-Cys-Gly
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 25000, SDS-PAGE, dimer also catalytically active Silene vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Silene vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 47 half-maximal activity at 20°C and 47°C Silene vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
 30% glycerol, 500 h, small activity decrease Silene vulgaris
4
-
 50% activity after 140 h Silene vulgaris
22
-
 50% activity after 34 h Silene vulgaris
35
-
 50% activity after 0.5 h Silene vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Silene vulgaris