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Literature summary for 2.3.2.15 extracted from

  • Chaurasia, N.; Mishra, Y.; Chatterjee, A.; Rai, R.; Yadav, S.; Rai, L.C.
    Overexpression of phytochelatin synthase (pcs) enhances abiotic stress tolerance by altering the proteome of transformed Anabaena sp. PCC 7120 (2017), Protoplasma, 254, 1715-1724 .
    View publication on PubMed
Search for more literature for 2.3.2.15

Cloned(Commentary)

Cloned (Comment) Organism
gene alr0975 or pcs, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain DH5alpha, and functional constitutive overexpression in Anabaena sp. strain PCC 7120, semiquantitative RT-PCR enzyme expression analysis Nostoc sp. PCC 7120 = FACHB-418

Protein Variants

Protein Variants Comment Organism
additional information an extra copy of phytochelatin-encoding gene phytochelatin synthase (pcs) is constitutively expressed in Anabaena sp. strain PCC7120 (An7120), an integrative expression vector pFPN is used for incorporating a cassette of genes for genomic integration and expression. Comparative growth behavior of wild-type strain and overexpressed strain AnFPN-pcs under LC50 doses of UV-B, salt, heat, copper, carbofuran, and cadmium resulted in decrement in specific growth rate by 26, 21, 27, 13, 30, and 17%, respectively, in An7120 compared to AnFPN-pcs thereby suggesting that the transformed cyanobacterium AnFPN-pcs has developed tolerance and hence shows better growth performances under different abiotic stresses. Comparison of differentially expressed proteins in An7120 and AnFPN-pcs, and dynamics of differentially expressed proteins in An7120 and AnFPN-pcs, overview Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly Nostoc sp. PCC 7120 = FACHB-418
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418 Q8YY76 SAG 25.82, UTEX 2576, i.e. Anabaena sp. PCC 7120
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
 Nostoc sp. PCC 7120 = FACHB-418 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Synonyms

Synonyms Comment Organism
alr0975
-
 Nostoc sp. PCC 7120 = FACHB-418
PCS
-
 Nostoc sp. PCC 7120 = FACHB-418
phytochelatin synthase
-
 Nostoc sp. PCC 7120 = FACHB-418

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Nostoc sp. PCC 7120 = FACHB-418

General Information

General Information Comment Organism
physiological function role of of enzyme PCS in abiotic stress tolerance Nostoc sp. PCC 7120 = FACHB-418