Literature summary for 2.3.2.15 extracted from

Zhang, D.; Yamamoto, T.; Tang, D.; Kato, Y.; Horiuchi, S.; Ogawa, S.; Yoshimura, E.; Suzuki, M.
Enhanced biosynthesis of CdS nanoparticles through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli with fluorescence effect in detection of pyrogallol and gallic acid (2019), Talanta, 195, 447-455 .

Search for more literature for 2.3.2.15

Application

Application	Comment	Organism
biotechnology	enhanced biosynthesis of cadmium(II)-selenide (CdS) nanoparticles (NPs) through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli with fluorescence effect in detection of pyrogallol and gallic acid. CdS NPs act as a classical II-VI semiconductor with various interesting properties and incomparable advantages. Enzyme PCS can effectively capture and enrich Cd2+ so that the generation and subsequent application process of CdS NPs will be greatly improved. A direct detection method based on such CdS NPs is established for determination of pyrogallol and gallic acid, two organic molecules with multiple advantages and wide applications. The method provides an excellent alternative to traditional chemosynthesis and quantitative analysis, which also displays great potential for technological improvement towards green synthesis and application. Method development and evaluation, detailed overview	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
gene PCS1, recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glutathione + [Glu(-Cys)]n-Gly	Arabidopsis thaliana	-	Gly + [Glu(-Cys)]n+1-Gly	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9S7Z3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + [Glu(-Cys)]n-Gly	-	Arabidopsis thaliana	Gly + [Glu(-Cys)]n+1-Gly	-	?
additional information	the PCS enzyme catalyzes transformation of GSH to give phytochelatins (PCs), it can effectively capture and enrich Cd2+ so that the generation and subsequent application process of CdS NPs is greatly improved	Arabidopsis thaliana	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 54500, recombinant enzyme, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AtPCS1	-	Arabidopsis thaliana
PCS	-	Arabidopsis thaliana
PCS1	-	Arabidopsis thaliana
phytochelatin synthase	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Arabidopsis thaliana

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	8	activity range	Arabidopsis thaliana

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Release 2023.1 (January 2023)