Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Maleate | stimulates hydrolase activity | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetazolamide Maleate | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bos taurus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 55000 + 1 * 25000, SDS-PAGE | Bos taurus |
55000 | - |
1 * 55000 + 1 * 25000, SDS-PAGE | Bos taurus |
80000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
Holstein breed | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | sialoglycoprotein | Bos taurus |
glycoprotein | both subunits are glycoproteins | Bos taurus |
glycoprotein | 20% carbohydrate | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid | mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colostrum | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
407 | - |
purified enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | best donor: glutamate | Bos taurus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptors are amino acids or dipeptides | Bos taurus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptor specificity, overview | Bos taurus | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate | beta-enantiomer of aminobutyrate | Bos taurus | 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine | - |
? | |
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate | alpha-enantiomer of aminobutyrate | Bos taurus | 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine | - |
? | |
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide | - |
Bos taurus | 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline | - |
? | |
5-L-glutamyl-4-nitroanilide + glycine | 19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors | Bos taurus | 4-nitroaniline + 5-L-glutamyl-glycine | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | best acceptor | Bos taurus | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-alanine | - |
Bos taurus | 4-nitroaniline + 5-L-glutamyl-L-alanine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamate | - |
Bos taurus | 4-nitroaniline + 5-L-glutamyl-L-glutamate | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamine | - |
Bos taurus | 4-nitroaniline + 5-L-glutamyl-L-glutamine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionine | best acceptor | Bos taurus | 4-nitroaniline + 5-L-glutamyl-L-methionine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-proline | - |
Bos taurus | 4-nitroaniline + 5-L-glutamyl-L-proline | - |
? | |
5-L-glutamyl-4-nitroanilide + L-serine | - |
Bos taurus | 4-nitroaniline + 5-L-glutamyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 55000 + 1 * 25000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 3.85 | Bos taurus |
8.5 | - |
with acceptor glycylglycine | Bos taurus |
9 | - |
broad | Bos taurus |
9 | - |
without acceptor | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.5 | about half-maximal activity at pH 7.3, maximal activity at pH 8.5 | Bos taurus |