Cloned (Comment) | Organism |
---|---|
expression of recombinant wild-type and mutants in Spodoptera frugiperda Sf21 cells via baculovirus infection | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H383A | site-directed mutagenesis of conserved His383 residue, 3fold reduced activity and 62% reduced Vmax, altered binding of acceptor | Homo sapiens |
H383A/H505A | site-directed mutagenesis, 37fold reduced activity | Homo sapiens |
H505A | site-directed mutagenesis, 10fold reduced activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Homo sapiens | |
0.008 | - |
L-Glu-4-nitroanilide | hydrolase reaction | Homo sapiens | |
1.4 | - |
L-Glu-4-nitroanilide | recombinant wild-type, transpeptidation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutants from Sf21 insect cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid | kinetic study | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
purified recombinant mutant H383A/H505A | Homo sapiens |
47 | - |
purified recombinant mutant H505A | Homo sapiens |
150 | - |
purified recombinant mutant H383A | Homo sapiens |
440 | - |
purified recombinant wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | - |
Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + H2O | hydrolase reaction | Homo sapiens | 4-nitroaniline + L-glutamate | - |
? | |
L-Glu-4-nitroanilide + glutathione | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |