Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effects by KCl, KNO3, KBr, NaCl, CaCl2, MgCl2, MnCl2, CoCl2, ZnCl2, DTT, EDTA | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by KCl, KNO3, KBr, NaCl, CaCl2, MgCl2, MnCl2, CoCl2, ZnCl2, DTT, EDTA | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | biphasic kinetics in transpeptidase reaction with 1-aminocyclopropane-1-carboxylic acid as acceptor, Km-values | Solanum lycopersicum | |
0.09 | - |
glutathione | isoform II | Solanum lycopersicum | |
0.11 | - |
glutathione | isoform I | Solanum lycopersicum | |
1.7 | - |
L-Glu-4-nitroanilide | isoform I, hydrolase reaction | Solanum lycopersicum | |
2.1 | - |
L-Glu-4-nitroanilide | isoform II, hydrolase reaction | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Solanum lycopersicum | 16020 | - |
peripheral membrane protein | isoforms I and II | Solanum lycopersicum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no effect | Solanum lycopersicum | |
K+ | no effect | Solanum lycopersicum | |
Mg2+ | no effect | Solanum lycopersicum | |
additional information | no effects by MnCl2, CoCl2, ZnCl2 | Solanum lycopersicum | |
Na+ | no effect | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | Solanum lycopersicum | concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor | peptide + 5-L-glutamyl amino acid | - |
? | |
glutathione + an amino acid | Solanum lycopersicum | - |
L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
2isoforms I and II | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | isoforms I and II | Solanum lycopersicum |
glycoprotein | binding of concanavalin A | Solanum lycopersicum |
Purification (Comment) | Organism |
---|---|
2 isoforms I and II | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pericarp | 2 isoforms I and II | Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.175 | - |
purified isoform II, transpeptidation | Solanum lycopersicum |
13.41 | - |
purified isoform I, transpeptidation | Solanum lycopersicum |
17.37 | - |
purified isoform II, hydrolase reaction | Solanum lycopersicum |
24.03 | - |
purified isoform I, hydrolase reaction | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: hydrolase reaction with H2O as acceptor | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: glutathione S-conjugates | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | alpha-aminoisobutyric acid is no donor | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptor specificity, overview | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is GSH | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: glutathione disulfide | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor specificity, overview | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptors: L-methionine, L-tryptophan, L-alanine, 1-aminocyclopropane-1-carboxylic acid, glutathione | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor | Solanum lycopersicum | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + H2O | hydrolase reaction | Solanum lycopersicum | 4-nitroaniline + L-glutamate | - |
? | |
glutathione + 1-aminocyclopropane-1-carboxylic acid | isoforms I and II | Solanum lycopersicum | L-cysteinylglycine + 5-L-glutamyl-1-aminocyclopropane-1-carboxylic acid | - |
? | |
glutathione + an amino acid | - |
Solanum lycopersicum | L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? | |
glutathione + H2O | hydrolase reaction, concurrent to (auto-)transpeptidation | Solanum lycopersicum | L-cysteinylglycine + L-glutamate | - |
? | |
L-Glu-4-nitroanilide + glutathione | - |
Solanum lycopersicum | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, isoforms I and II, SDS-PAGE | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
gamma-GTase | - |
Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | hydrolysis and transpeptidation, isoforms I and II | Solanum lycopersicum |