Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.2 extracted from

  • Chu, L.; Xu, X.; Dong, Z.; Cappelli, D.; Ebersole, J.L.
    Role for recombinant gamma-glutamyltransferase from Treponema denticola in glutathione metabolism (2003), Infect. Immun., 71, 335-342.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Treponema denticola
dithiothreitol activation Treponema denticola

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Treponema denticola

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by benzamidine Treponema denticola
Nalpha-4-tosyl-L-lysine chloromethyl ketone inactivation Treponema denticola
PMSF no effect Treponema denticola
Pronase inactivation Treponema denticola
proteinase K inactivation Treponema denticola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Treponema denticola
additional information
-
 amino acid sequence Treponema denticola
31000
-
 x * 31000, SDS-PAGE Treponema denticola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ Treponema denticola
-
 peptide + 5-L-glutamyl amino acid
-
 ?
glutathione + an amino acid Treponema denticola initial step of glutathione degradation L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?
additional information Treponema denticola purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium ?
-
 ?
additional information Treponema denticola L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
 strain ATCC 35405
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from E. coli Treponema denticola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
158.4
-
 purified enzyme Treponema denticola

Storage Stability

Storage Stability Organism
-20°C, stable Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
 Treponema denticola peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ L-cysteine is no substrate Treponema denticola peptide + 5-L-glutamyl amino acid
-
 ?
5-D-glutamyl-4-nitroanilide + acceptor
-
 Treponema denticola 4-nitroaniline + 5-D-glutamyl-acceptor
-
 ?
glutathione + an amino acid initial step of glutathione degradation Treponema denticola L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?
glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Treponema denticola L-cysteinylglycine + L-glutamate
-
 ?
additional information purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium Treponema denticola ?
-
 ?
additional information L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate Treponema denticola ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Treponema denticola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 inactivation Treponema denticola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.9 Treponema denticola