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Literature summary for 2.3.2.2 extracted from

  • Wu, Q.; Xu, H.; Zhang, L.; Yao, J.; Ouyang, P.
    Production, purification and properties of gamma-glutamyltranspeptidase from a newly isolated Bacillus subtilis NX-2 (2006), J. Mol. Catal. B, 43, 113-117.
No PubMed abstract available
Search for more literature for 2.3.2.2

Application

Application Comment Organism
synthesis the enzyme can catalyze the transfer of L-glutamine to Gly-Gly to synthesize Gln-Gly-Gly, which is promising for the synthesis of valuable gamma-glutamyl peptides Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 1 * 32000 + 1 * 43000, SDS-PAGE Bacillus subtilis
43000
-
 1 * 32000 + 1 * 43000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis NX-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
73.36
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + Arg 113.6% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
5-L-glutamyl-4-nitroanilide + Arg 113.6% of the activity with Gly-Gly Bacillus subtilis NX-2 4-nitroaniline + 5-L-glutamyl-Arg
-
 ?
5-L-glutamyl-4-nitroanilide + Asn 39.3% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Asn
-
 ?
5-L-glutamyl-4-nitroanilide + Asn 39.3% of the activity with Gly-Gly Bacillus subtilis NX-2 4-nitroaniline + 5-L-glutamyl-Asn
-
 ?
5-L-glutamyl-4-nitroanilide + Asp 4.5% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
5-L-glutamyl-4-nitroanilide + Asp 4.5% of the activity with Gly-Gly Bacillus subtilis NX-2 4-nitroaniline + 5-L-glutamyl-Asp
-
 ?
5-L-glutamyl-4-nitroanilide + Cys 46.9% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Cys
-
 ?
5-L-glutamyl-4-nitroanilide + DL-Phe 39.9% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-DL-Phe
-
 ?
5-L-glutamyl-4-nitroanilide + Glu 4.4% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Glu
-
 ?
5-L-glutamyl-4-nitroanilide + Gly 33.2% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Gly 33.2% of the activity with Gly-Gly Bacillus subtilis NX-2 4-nitroaniline + 5-L-glutamyl-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Bacillus subtilis NX-2 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?
5-L-glutamyl-4-nitroanilide + His 82.9% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-His
-
 ?
5-L-glutamyl-4-nitroanilide + Ile 20.7% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Ile
-
 ?
5-L-glutamyl-4-nitroanilide + L-tyrosine 24.1% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-L-tyrosine
-
 ?
5-L-glutamyl-4-nitroanilide + Leu 21.3% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Leu
-
 ?
5-L-glutamyl-4-nitroanilide + Lys 79.4% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Lys
-
 ?
5-L-glutamyl-4-nitroanilide + Met 48.8% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Met
-
 ?
5-L-glutamyl-4-nitroanilide + Pro 13.8% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Pro
-
 ?
5-L-glutamyl-4-nitroanilide + Ser 12.8% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Ser
-
 ?
5-L-glutamyl-4-nitroanilide + Thr 27.3% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Thr
-
 ?
5-L-glutamyl-4-nitroanilide + Tyr 34.0% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Tyr
-
 ?
5-L-glutamyl-4-nitroanilide + Val 30.2% of the activity with Gly-Gly Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-Val
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 32000 + 1 * 43000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 70% of maximal activity, 60°C: about 90% of maximal activity, 70°C: about 40% of maximal activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 40% of maximal activity, pH 9.0: 65% of maximal activity Bacillus subtilis