Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | 1 mM, complete inhibition | Bacillus pumilus | |
Co2+ | slight inhibition | Bacillus pumilus | |
EDTA | 1 mM, 35% inhibition | Bacillus pumilus | |
EGTA | 1 mM, 28% inhibition | Bacillus pumilus | |
iodoacetate | 1 mM, 9% inhibition | Bacillus pumilus | |
L-Ser | 10 mM, 26% inhibition | Bacillus pumilus | |
pepstatin | 0.0005 mM, 9% inhibition | Bacillus pumilus | |
PMSF | 1 mM, 11% inhibition | Bacillus pumilus | |
Zn2+ | slight inhibition | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
5-L-glutamyl-4-nitroanilide | hydrolysis | Bacillus pumilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus pumilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 38000 + 1 * 23000, only the large subunit is active when the enzyme is dissociated under denaturing conditions. The behavior of the native enzyme suggests that the active site of the large subunit is masked by the small subunit, SDS-PAGE | Bacillus pumilus |
38000 | - |
1 * 38000 + 1 * 23000, only the large subunit is active when the enzyme is dissociated under denaturing conditions. The behavior of the native enzyme suggests that the active site of the large subunit is masked by the small subunit, SDS-PAGE | Bacillus pumilus |
61000 | - |
gel filtration | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
originating from Lake Bogoria | - |
Bacillus pumilus A8 | - |
originating from Lake Bogoria | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus pumilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Bacillus pumilus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala | - |
? | |
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala | - |
Bacillus pumilus A8 | 4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala | - |
? | |
5-L-glutamyl-4-nitroanilide + Arg | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Arg | - |
Bacillus pumilus A8 | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus pumilus A8 | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala | - |
Bacillus pumilus A8 | 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly | - |
Bacillus pumilus A8 | 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + H2O | - |
Bacillus pumilus | L-glutamate + 4-nitroaniline | - |
? | |
5-L-glutamyl-4-nitroanilide + Ile | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Ile | - |
? | |
5-L-glutamyl-4-nitroanilide + Lys | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Lys | - |
? | |
5-L-glutamyl-4-nitroanilide + Met | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Met | - |
? | |
5-L-glutamyl-4-nitroanilide + Thr | - |
Bacillus pumilus | 4-nitroaniline + 5-L-glutamyl-Thr | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 38000 + 1 * 23000, only the large subunit is active when the enzyme is dissociated under denaturing conditions. The behavior of the native enzyme suggests that the active site of the large subunit is masked by the small subunit, SDS-PAGE | Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | - |
- |
Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
10 min, completely stable | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9 | reaction with Gly-Gly as acceptor | Bacillus pumilus |