Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.2 extracted from

  • Moallic, C.; Dabonne, S.; Colas, B.; Sine, J.P.
    Identification and characterization of a gamma-glutamyl transpeptidase from a thermo-alcalophile strain of Bacillus pumilus (2006), Protein J., 25, 391-397.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine 1 mM, complete inhibition Bacillus pumilus
Co2+ slight inhibition Bacillus pumilus
EDTA 1 mM, 35% inhibition Bacillus pumilus
EGTA 1 mM, 28% inhibition Bacillus pumilus
iodoacetate 1 mM, 9% inhibition Bacillus pumilus
L-Ser 10 mM, 26% inhibition Bacillus pumilus
pepstatin 0.0005 mM, 9% inhibition Bacillus pumilus
PMSF 1 mM, 11% inhibition Bacillus pumilus
Zn2+ slight inhibition Bacillus pumilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
5-L-glutamyl-4-nitroanilide hydrolysis Bacillus pumilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus pumilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
1 * 38000 + 1 * 23000, only the large subunit is active when the enzyme is dissociated under denaturing conditions. The behavior of the native enzyme suggests that the active site of the large subunit is masked by the small subunit, SDS-PAGE Bacillus pumilus
38000
-
1 * 38000 + 1 * 23000, only the large subunit is active when the enzyme is dissociated under denaturing conditions. The behavior of the native enzyme suggests that the active site of the large subunit is masked by the small subunit, SDS-PAGE Bacillus pumilus
61000
-
gel filtration Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus
-
originating from Lake Bogoria
-
Bacillus pumilus A8
-
originating from Lake Bogoria
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus pumilus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Bacillus pumilus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
-
Bacillus pumilus A8 4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
-
?
5-L-glutamyl-4-nitroanilide + Arg
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Arg
-
?
5-L-glutamyl-4-nitroanilide + Arg
-
Bacillus pumilus A8 4-nitroaniline + 5-L-glutamyl-Arg
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
Bacillus pumilus A8 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
-
Bacillus pumilus A8 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
-
Bacillus pumilus A8 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
-
?
5-L-glutamyl-4-nitroanilide + H2O
-
Bacillus pumilus L-glutamate + 4-nitroaniline
-
?
5-L-glutamyl-4-nitroanilide + Ile
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Ile
-
?
5-L-glutamyl-4-nitroanilide + Lys
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Lys
-
?
5-L-glutamyl-4-nitroanilide + Met
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Met
-
?
5-L-glutamyl-4-nitroanilide + Thr
-
Bacillus pumilus 4-nitroaniline + 5-L-glutamyl-Thr
-
?

Subunits

Subunits Comment Organism
dimer 1 * 38000 + 1 * 23000, only the large subunit is active when the enzyme is dissociated under denaturing conditions. The behavior of the native enzyme suggests that the active site of the large subunit is masked by the small subunit, SDS-PAGE Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
62
-
-
Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
10 min, completely stable Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9 reaction with Gly-Gly as acceptor Bacillus pumilus