Protein Variants | Comment | Organism |
---|---|---|
H320G | effect of H320G substitution, solvent accessibility, and conformational changes on catalysis and activity | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
acetyl-CoA | wild-type | Sus scrofa | |
0.0059 | - |
oxaloacetate | wild-type | Sus scrofa | |
0.043 | - |
oxaloacetate | mutant H320G | Sus scrofa | |
0.089 | - |
acetyl-CoA | mutant H320G | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | mechanism | Sus scrofa | |
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | active site His320 | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | intermediate citryl-CoA | Sus scrofa | citrate + CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | molecular basis of subunit interactions | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.275 | - |
oxaloacetate | mutant H320G | Sus scrofa | |
167 | - |
oxaloacetate | wild-type | Sus scrofa |