Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | - |
Gallus gallus | |
acetoacetyl-CoA | - |
Rattus norvegicus | |
Mg2+ | mitochondrial enzyme | Gallus gallus | |
palmitoyl-CoA | mitochondrial synthase | Gallus gallus | |
succinyl-CoA | mitochondrial synthase, 50% inhibition | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
acetoacetyl-CoA | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
cytoplasm | distinct mitochondrial and cytoplasmic forms | Gallus gallus | 5737 | - |
cytosol | - |
Gallus gallus | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | distinct mitochondrial and cytoplasmic forms | Gallus gallus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cytoplasmic enzyme | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Gallus gallus |
57000 | - |
2 * 57000, gel filtration | Gallus gallus |
96000 | - |
gel filtration | Gallus gallus |
105000 | - |
sedimentation equlibrium | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Rattus norvegicus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
chicken | - |
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
mitochondrial form | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-20°C, purified synthase may be stored for 2-3 months with little loss of activity by adding 30% glyerol | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Rattus norvegicus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis | Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Gallus gallus |
dimer | 2 * 57000, gel filtration | Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
palmityl-CoA | - |
Gallus gallus |