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Literature summary for 2.3.3.10 extracted from

  • Reed, W.D.; Lane, M.D.
    Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase from chicken liver (1975), Methods Enzymol., 35 B, 155-160.
No PubMed abstract available
Search for more literature for 2.3.3.10

General Stability

General Stability Organism
relatively stable dialyzed against 10 mM potassium phosphate in 0.1 mM dithiothreitol and EDTA for 4-10 days Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA
-
 Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 acetyl-CoA 0.005 mM acetoacetyl-CoA Gallus gallus
0.5
-
 acetyl-CoA 0.05 mM acetoacetyl-CoA Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 2 * 53000, SDS-PAGE Gallus gallus
105000
-
 sedimentation equlibrium Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus potentially favorable position for the regulation of hepatic ketogenesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 chicken
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, may be stored in 30% glycerin solution for 2-3 months with no loss of activity Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O potentially favorable position for the regulation of hepatic ketogenesis Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Gallus gallus