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Literature summary for 2.3.3.10 extracted from

  • Lowe, D.M.; Tubbs, P.K.
    3-Hydroxy-3-methylglutaryl-coenzyme A synthase from ox liver (1985), Biochem. J., 227, 591-599.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.10

General Stability

General Stability Organism
can be rapidly frozen in solid CO2/acetone and freeze-dried for long-term storage, not stable to freezing and thawing in absence of glycerol Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA competitive inhibition Bos taurus
acetoacetyl-CoA
-
 Gallus gallus
acetoacetyl-CoA
-
 Saccharomyces cerevisiae
CoA mixed product inhibition Bos taurus
CoA
-
 Saccharomyces cerevisiae
DL-3-hydroxy-3-methylglutaryl-CoA mixed product inhibition Bos taurus
DL-3-hydroxy-3-methylglutaryl-CoA
-
 Gallus gallus
DL-3-hydroxy-3-methylglutaryl-CoA
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Gallus gallus 5737
-
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
cytoplasm
-
 Bos taurus 5737
-
mitochondrion
-
 Gallus gallus 5739
-
mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47900
-
 2 * 47900, SDS-PAGE Bos taurus
94600
-
 sedimentation equilibrium Bos taurus
100000
-
-
 Gallus gallus
100000
-
-
 Bos taurus
111000
-
 gel filtration Bos taurus
119300
-
 sedimentation velocity and light-scattering Bos taurus
130000
-
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Bos taurus pathway of ketogenesis in the mitochondria, mevalonate synthesis in the cytoplasm (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 ox
-
Gallus gallus
-
 chicken
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.88
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-20°C, stored in presence of 30% glycerol and 5 mM dithiothreitol, remains active for several months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Bos taurus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O pathway of ketogenesis in the mitochondria, mevalonate synthesis in the cytoplasm Bos taurus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Gallus gallus
dimer 2 * 47900, SDS-PAGE Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0417
-
 acetyl-CoA
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0035
-
 acetoacetyl-CoA
-
 Bos taurus