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Literature summary for 2.3.3.10 extracted from

  • Misra, I.; Charlier, H.A.; Miziorko, H.M.
    Avian cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase. Evaluation of the role of cysteines in reaction chemistry (1995), Biochim. Biophys. Acta, 1247, 253-259.
    View publication on PubMed
Search for more literature for 2.3.3.10

Cloned(Commentary)

Cloned (Comment) Organism
expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.274
-
 acetyl-CoA mutant C59A Gallus gallus
0.28
-
 acetyl-CoA mutant C69A Gallus gallus
0.294
-
 acetyl-CoA wild-type Gallus gallus
0.355
-
 acetyl-CoA mutant C232A Gallus gallus
0.435
-
 acetyl-CoA mutant C268A Gallus gallus
1.561
-
 acetyl-CoA mutant C224A Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Gallus gallus 5829
-
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Blattella germanica 5829
-
cytosol
-
 Cricetulus griseus 5829
-
mitochondrion
-
 Gallus gallus 5739
-
mitochondrion
-
 Mus musculus 5739
-
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Mus musculus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Blattella germanica
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Cricetulus griseus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus produces a key intermediate in steroidogenic and ketogenic metabolic pathways (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Blattella germanica P54870 cockroach, cytosolic isoform 2
-
Blattella germanica P54961 cockroach, cytosolic isoform 1
-
Cricetulus griseus P13704 chinese hamster, cytosolic isoform
-
Gallus gallus
-
 chicken
-
Gallus gallus
-
 avian
-
Homo sapiens
-
 human
-
Mus musculus
-
 mouse
-
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mus musculus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Cricetulus griseus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O produces a key intermediate in steroidogenic and ketogenic metabolic pathways Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
7.75 9.5
-
 Gallus gallus