Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | 5 mM, activates | Brassica juncea |
Cloned (Comment) | Organism |
---|---|
His6-tagged protein from Escherichia coli,His6-BjHMGS1 | Brassica juncea |
Protein Variants | Comment | Organism |
---|---|---|
C212C | mutation causes 6fold decreased maximal velocity | Brassica juncea |
H188N | mutation causes 8fold decreased maximal velocity, mutant enzyme does not display substrate inhibition by acetoacetyl-CoA | Brassica juncea |
H188N/S359A | 10fold increased specific activity, no inhibition by acetoacetyl-CoA | Brassica juncea |
N115A | KI value for acetoacetyl-CoA is 2.3fold higher than the wild-type value, the turnover number for acetyl-CoA is 17.7fold lower than wild-type value, KM-value is 1.6fold lower than wild-type value | Brassica juncea |
R157A | mutation causes 14fold decreased maximal velocity | Brassica juncea |
S356A | KI value for acetoacetyl-CoA is 5.2fold higher than the wild-type value, the turnover number for acetyl-CoA is 25.8fold lower than wild-type value, KM-value is 1.6fold lower than wild-type value | Brassica juncea |
S359A | 10fold increased specific activity | Brassica juncea |
S89A | KI value for acetoacetyl-CoA is 1.2fold higher than the wild-type value, the turnover number for acetyl-CoA is 54.6fold lower than wild-type value, KM-value is 2.3fold lower than wild-type value | Brassica juncea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA | - |
Brassica juncea | |
acetoacetyl-CoA | inhibition of wild-type enzyme, no inhibition of mutant enzyme H188N and H188N/S259A | Brassica juncea | |
Co2+ | - |
Brassica juncea | |
CoA | - |
Brassica juncea | |
F-244 | IC50: 35 nM | Brassica juncea | |
Mn2+ | - |
Brassica juncea | |
Zn2+ | - |
Brassica juncea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme H188N/S359A | Brassica juncea | |
0.012 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme H188N | Brassica juncea | |
0.018 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme S89A | Brassica juncea | |
0.024 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme S359A | Brassica juncea | |
0.026 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme S356A | Brassica juncea | |
0.027 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme N115A | Brassica juncea | |
0.029 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme C212S | Brassica juncea | |
0.043 | - |
acetyl-CoA | pH 7.5, 30°C, His6-BjHMGS1 wild-type enzyme | Brassica juncea | |
0.052 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme R157A | Brassica juncea | |
0.055 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme H188N | Brassica juncea | |
0.12 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme H188N/S359A | Brassica juncea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no stimulation by Mg2+, Mn2+, Zn2+ and Co2+ | Brassica juncea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Brassica juncea | second enzyme in the cytoplasmic mevalonate pathway of isoprenoid biosynthesis | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
- |
Brassica juncea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Brassica juncea | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | second enzyme in the cytoplasmic mevalonate pathway of isoprenoid biosynthesis | Brassica juncea | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Brassica juncea |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl-CoA synthase 1 | - |
Brassica juncea |
HMG-CoA synthase 1 | - |
Brassica juncea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Brassica juncea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | about 70% of maximal activity at 30°C and at 45°C | Brassica juncea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme R157A | Brassica juncea | |
0.0029 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme H188N | Brassica juncea | |
0.0041 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme C212S | Brassica juncea | |
0.0076 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme S89A | Brassica juncea | |
0.0161 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme S356A | Brassica juncea | |
0.0234 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme N115A | Brassica juncea | |
0.214 | - |
acetyl-CoA | pH 7.5, 30°C, mutant enzyme S359A | Brassica juncea | |
0.415 | - |
acetyl-CoA | pH 7.5, 30°C, His6-BjHMGS1 wild-type enzyme | Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Brassica juncea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 60% of maximal activity, pH 9.5: about 30% of maximal activity | Brassica juncea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 7.5, 30°C, wild-type enzyme | Brassica juncea | |
0.028 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme S359A | Brassica juncea | |
0.034 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 7.5, 30°C, mutant enzyme H188N | Brassica juncea | |
0.038 | - |
acetoacetyl-CoA | pH 7.5, 30°C, wild-type enzyme | Brassica juncea | |
0.0389 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 7.5, 30°C, mutant enzyme H188N/S359A | Brassica juncea | |
0.039 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme C212S | Brassica juncea | |
0.046 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme S89A | Brassica juncea | |
0.08 | - |
CoA | pH 7.5, 30°C, wild-type enzyme | Brassica juncea | |
0.091 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme N115A | Brassica juncea | |
0.199 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme S356A | Brassica juncea | |
0.242 | - |
acetoacetyl-CoA | pH 7.5, 30°C, mutant enzyme R157A | Brassica juncea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000035 | - |
IC50: 35 nM | Brassica juncea | F-244 |