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Literature summary for 2.3.3.14 extracted from

  • Gabriel, I.; Milewski, S.
    Characterization of recombinant homocitrate synthase from Candida albicans (2016), Protein Expr. Purif., 125, 7-18.
    View publication on PubMed
Search for more literature for 2.3.3.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
2-aminoethyl-L-cysteine
-
 Candida albicans
Cu2+ strong inhibitory effect on isoform Lys22p Candida albicans
DL-5-hydroxylysine
-
 Candida albicans
DL-alpha-Difluoromethyllysine strong inhibitor of isoform Lys22p, but not Lys21p Candida albicans
EDTA strong inhibitory effect on isoform Lys22p Candida albicans
L-Arg inhibitor of isoform Lys22p, but not Lys21p Candida albicans
L-Lys competitive inhibitor Candida albicans
additional information not inhibited by L-ornithine, L-norleucine, L-leucine, L-canavanine, gamma-amino-beta-hydroxybutyric acid, gamma-amino-n-butyric acid, and O-(2-aminoethyl)-L-serine; there is little or no effect of Mg2+ or Ca2+ ions Candida albicans
S-(2-aminoethyl)-L-cysteine
-
 Candida albicans
Zn2+ strong inhibitory effect on isoform Lys22p Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.113
-
 2-oxoglutarate isoform Lys21p, at pH 7.5 and 22°C Candida albicans
0.152
-
 2-oxoglutarate isoform Lys22p, at pH 7.5 and 22°C Candida albicans
0.48
-
 acetyl-CoA isoform Lys22p, at pH 7.5 and 22°C Candida albicans
0.8
-
 acetyl-CoA isoform Lys21p, at pH 7.5 and 22°C Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47087
-
 4 * 47087, isoform Lys22p, calculated from amino acid sequence Candida albicans
48300
-
 4 * 48300, isoform Lys21p, SDS-PAGE Candida albicans
48314
-
 4 * 48314, isoform Lys21p, calculated from amino acid sequence Candida albicans
49400
-
 4 * 49400, isoform Lys22p, SDS-PAGE Candida albicans
200700
-
 isoform Lys22p, gel filtration Candida albicans
210300
-
 isoform Lys21p, gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate Candida albicans
-
 (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate
-
 Candida albicans (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?
additional information isoforms Lys21p and Lys22p are not able to catalyze the condensation of oxaloacetate with acetyl-CoA to give citrate Candida albicans ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 47087, isoform Lys22p, calculated from amino acid sequence Candida albicans
homotetramer 4 * 48300, isoform Lys21p, SDS-PAGE Candida albicans
homotetramer 4 * 48314, isoform Lys21p, calculated from amino acid sequence Candida albicans
homotetramer 4 * 49400, isoform Lys22p, SDS-PAGE Candida albicans

Synonyms

Synonyms Comment Organism
HCS
-
 Candida albicans
LYS21
-
 Candida albicans
Lys21p isoform Candida albicans
LYS22
-
 Candida albicans
Lys22p isoform Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 acetyl-CoA isoform Lys21p, at pH 7.5 and 22°C Candida albicans
0.2
-
 acetyl-CoA isoform Lys22p, at pH 7.5 and 22°C Candida albicans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.032
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans DL-alpha-Difluoromethyllysine
0.0541
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans L-Lys
0.286
-
 isoform Lys21p, at pH 7.5 and 22°C Candida albicans L-Lys
1.8
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans S-(2-aminoethyl)-L-cysteine
5.7
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans DL-5-hydroxylysine
16.5
-
 isoform Lys21p, at pH 7.5 and 22°C Candida albicans 2-aminoethyl-L-cysteine
18.7
-
 isoform Lys22p, at pH 7.5 and 22°C Candida albicans L-Arg