Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | activates at 0.5 mM | Dictyostelium discoideum |
General Stability | Organism |
---|---|
destabilization by all salts, exception is ammonium sulfate, which stabilizes at low concentration | Dictyostelium discoideum |
dithiothreitol stabilizes for about 5 days | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | no inhibition | Dictyostelium discoideum | |
acetyl-CoA | no inhibition | Dictyostelium discoideum | |
ATP | no inhibition | Dictyostelium discoideum | |
CoA | competitive with acetyl-CoA, noncompetitive with oxaloacetate | Dictyostelium discoideum | |
NADH | no effect | Dictyostelium discoideum | |
oxaloacetate | no inhibition | Dictyostelium discoideum | |
p-chloromercuribenzoate | can be restored by dithiothreitol treatment | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
oxaloacetate | - |
Dictyostelium discoideum | |
0.01 | - |
acetyl-CoA | - |
Dictyostelium discoideum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | activates at at least 0.5 mM | Dictyostelium discoideum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | Dictyostelium discoideum | key enzyme of tricarboxylic acid cycle | citrate + CoA | - |
r | |
acetyl-CoA + oxaloacetate + H2O | Dictyostelium discoideum NC-4 / ATCC 24697 | key enzyme of tricarboxylic acid cycle | citrate + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Dictyostelium discoideum NC-4 / ATCC 24697 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Dictyostelium discoideum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | ordered sequential mechanism | Dictyostelium discoideum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
471.7 | - |
partially purified enzyme | Dictyostelium discoideum |
Storage Stability | Organism |
---|---|
0°C, below, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol and 5 mg/ml bovine serum albumin, several months | Dictyostelium discoideum |
4°C, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol, 1 day | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Dictyostelium discoideum | citrate + CoA | - |
r | |
acetyl-CoA + oxaloacetate + H2O | key enzyme of tricarboxylic acid cycle | Dictyostelium discoideum | citrate + CoA | - |
r | |
acetyl-CoA + oxaloacetate + H2O | - |
Dictyostelium discoideum NC-4 / ATCC 24697 | citrate + CoA | - |
r | |
acetyl-CoA + oxaloacetate + H2O | key enzyme of tricarboxylic acid cycle | Dictyostelium discoideum NC-4 / ATCC 24697 | citrate + CoA | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
enzyme assay at | Dictyostelium discoideum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9 | - |
Dictyostelium discoideum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
CoA | - |
Dictyostelium discoideum |