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Literature summary for 2.3.3.16 extracted from

  • Higa, A.I.; Massarini, E.; Cazzulo, J.J.
    Purification and some properties of the citrate synthase from a marine Pseudomonas (1978), Can. J. Microbiol., 24, 215-221.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
AMP CS II, counteracted by NADH Pseudomonas sp.

General Stability

General Stability Organism
CS II much more stable than CS I Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) inactivation, half-lifes at 0.4 mM: 2.8 min for CS 1, 50 min for CS II Pseudomonas sp.
ADP CS II insensitive, CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate Pseudomonas sp.
AMP CS II insensitive, CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate Pseudomonas sp.
ATP CS II insensitive, CS I inhibited Pseudomonas sp.
KCl CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate Pseudomonas sp.
NADH CS II inhibited, CS II insensitive; weak inhibition, reversible by AMP, hinders the activation by AMP, CS II Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
oxaloacetate CS I Pseudomonas sp.
0.035
-
acetyl-CoA CS I Pseudomonas sp.
0.05
-
oxaloacetate CS II Pseudomonas sp.
0.45
-
acetyl-CoA CS II Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
Pseudomonas sp.
phosphate
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, SDS-PAGE, CS I, reassociation to CS II possible Pseudomonas sp.
53000
-
6 * 53000, SDS-PAGE, CS II Pseudomonas sp.
100000
-
gel filtration Pseudomonas sp.
100000
-
CS I Pseudomonas sp.
300000
-
CS II, gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
marine, 2 enzyme forms with different molecular weight, kinetic and regulatory properties, dissociated CS I /native CS II
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
102.7
-
purified enzyme Pseudomonas sp.

Storage Stability

Storage Stability Organism
4°C, Tris-HCl buffer, pH 7.6, 0.2 M KCl, 20% loss of activity after 1 month, only CS II Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Pseudomonas sp. citrate + CoA
-
?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE, CS I, reassociation to CS II possible Pseudomonas sp.
hexamer 6 * 53000, SDS-PAGE, CS II Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
enzyme assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
enzyme assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
AMP only CS II, counteracted by NADP Pseudomonas sp.