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Literature summary for 2.3.3.16 extracted from
- Citrate synthase purified from Tetrahymena mitochondria is identical with Tetrahymena 14-nm filament protein (1995), J. Biochem., 118, 189-195.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Tetrahymena pyriformis |  |
| additional information | inhibited by a specific antibody against 49K protein | Tetrahymena pyriformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0283 | - |
oxaloacetate | - |
Tetrahymena pyriformis | |
| 0.0286 | - |
acetyl-CoA | - |
Tetrahymena pyriformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Tetrahymena pyriformis | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | maximum activity at 150 mM | Tetrahymena pyriformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
x * 49000, SDS-PAGE | Tetrahymena pyriformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | Tetrahymena pyriformis | also acting as structural protein in oral morphogenesis and conjugation | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | Tetrahymena pyriformis W | also acting as structural protein in oral morphogenesis and conjugation | citrate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena pyriformis | - |
- |
- |
| Tetrahymena pyriformis | - |
identical with 14-nm filament protein, i.e. 49K protein | - |
| Tetrahymena pyriformis W | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tetrahymena pyriformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35.1 | - |
purified enzyme | Tetrahymena pyriformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Tetrahymena pyriformis | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | also acting as structural protein in oral morphogenesis and conjugation | Tetrahymena pyriformis | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Tetrahymena pyriformis W | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | also acting as structural protein in oral morphogenesis and conjugation | Tetrahymena pyriformis W | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, SDS-PAGE | Tetrahymena pyriformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Tetrahymena pyriformis |
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Release 2023.1 (January 2023)
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