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Literature summary for 2.3.3.16 extracted from
- Studies on yeast peroxisomal citrate synthase (1991), Arch. Biochem. Biophys., 286, 132-137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of peroxisomal isozyme CS II in citrate synthase deficient Escherichia coli mutant and wild-type Escherichia coli strain | Saccharomyces cerevisiae |
General Stability
| General Stability | Organism |
|---|---|
| peroxisomal enzyme protected by oxaloacetate | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Saccharomyces cerevisiae |  |
| AMP | only CS II | Saccharomyces cerevisiae | |
| ATP | strong inhibition | Saccharomyces cerevisiae | |
| additional information | no inhibition by NAD+ | Saccharomyces cerevisiae | |
| NADH | 51% inhibition of peroxisomal isozyme, 12% inhibition of mitochondrial isozyme at 5 mM | Saccharomyces cerevisiae | |
| NADP+ | only CS II slightly | Saccharomyces cerevisiae | |
| NADPH | 40% inhibition by 5 mM, both isoenzymes | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
oxaloacetate | mitochondrial isozyme CS I | Saccharomyces cerevisiae | |
| 0.004 | - |
acetyl-CoA | mitochondrial isozyme CS I | Saccharomyces cerevisiae | |
| 0.006 | 0.007 | oxaloacetate | native and recombinant peroxisomal isozyme CS II | Saccharomyces cerevisiae | |
| 0.011 | - |
acetyl-CoA | native peroxisomal isozyme CS II | Saccharomyces cerevisiae | |
| 0.013 | 0.014 | acetyl-CoA | recombinant peroxisomal isozyme CS II | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | 45% of CS II is bound to mitochondrial inner membrane and 2% of CS I | Saccharomyces cerevisiae | 5743 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
| peroxisome | - |
Saccharomyces cerevisiae | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, CS II, SDS-PAGE | Saccharomyces cerevisiae |
| 100000 | - |
gel filtration | Saccharomyces cerevisiae |
| 100000 | - |
CS II | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
peroxisomal isozyme CS II and mitochondrial isozyme CS I | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant peroxisomal isozyme CS II from E. coli | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified recombinant isozyme CS II | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Tris-HCl buffer, pH 7.4, 50% glycerol, 1 month | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Saccharomyces cerevisiae | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, CS II, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
both mitochondrial and peroxisomal enzymes | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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