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Literature summary for 2.3.3.16 extracted from
- Chimeric allosteric citrate synthases: construction and properties of citrate synthases containing domains from two different enzymes (1992), Arch. Biochem. Biophys., 298, 238-246.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of a chimeric protein with one Acinetobacter domain in Escherichia coli, domain interactions, subunit interactions | Escherichia coli |
| expression of a chimeric protein with one Escherichia coli domain in Escherichia coli, domain interactions, subunit interactions | Acinetobacter calcoaceticus subsp. anitratus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | chimeric proteins | Escherichia coli |
| additional information | chimeric proteins | Acinetobacter calcoaceticus subsp. anitratus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | allosteric | Acinetobacter calcoaceticus subsp. anitratus |  |
| NADH | allosteric | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
oxaloacetate | wild-type | Acinetobacter calcoaceticus subsp. anitratus | |
| 0.02 | - |
oxaloacetate | wild-type | Escherichia coli | |
| 0.11 | - |
acetyl-CoA | wild-type | Acinetobacter calcoaceticus subsp. anitratus | |
| 0.7 | - |
acetyl-CoA | wild-type | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | mutants, overview | Escherichia coli | |
| KCl | mutants, overview | Acinetobacter calcoaceticus subsp. anitratus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
alignment of partial amino acid sequence | Escherichia coli |
| additional information | - |
alignment of partial amino acid sequence | Acinetobacter calcoaceticus subsp. anitratus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus subsp. anitratus | - |
- |
- |
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant chimeric protein from E. coli | Escherichia coli |
| recombinant chimeric protein from E. coli | Acinetobacter calcoaceticus subsp. anitratus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
influence of KCl on wild-type and mutants | Escherichia coli |
| additional information | - |
influence of KCl on wild-type and mutants | Acinetobacter calcoaceticus subsp. anitratus |
| 45 | - |
purified wild-type enzyme | Escherichia coli |
| 57 | - |
purified recombinant chimeric Acinetobacter-type protein with small E. coli domain | Acinetobacter calcoaceticus subsp. anitratus |
| 88 | - |
purified wild-type enzyme | Acinetobacter calcoaceticus subsp. anitratus |
| 120 | - |
purified recombinant chimeric E. coli-type protein with small Acinetobacter domain | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Escherichia coli | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | - |
Acinetobacter calcoaceticus subsp. anitratus | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | recombinant chimeric enzymes, domain functions, subunit organisation | Escherichia coli |
| More | recombinant chimeric enzymes, domain functions, subunit organisation | Acinetobacter calcoaceticus subsp. anitratus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | chimeric mutants, overview | Escherichia coli | |
| additional information | - |
additional information | chimeric mutants, overview | Acinetobacter calcoaceticus subsp. anitratus | |
| 78 | - |
acetyl-CoA | wild-type, + 0.1 M KCl | Acinetobacter calcoaceticus subsp. anitratus | |
| 81 | - |
acetyl-CoA | wild-type, + 0.1 M KCl | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
recombinant chimeric protein | Escherichia coli |
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