Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.16 extracted from

  • Schmidtmann, E.; Koenig, A.C.; Orwat, A.; Leister, D.; Hartl, M.; Finkemeier, I.
    Redox regulation of Arabidopsis mitochondrial citrate synthase (2014), Mol. Plant, 7, 156-169.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
thioredoxin a strong and more than 8fold activation of enzyme activity is observed for isoform CS4 with Escherichia coli thioredoxin coupled to reduction by NTR and NADPH Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
H2O2 about 54% inhibition at 0.4 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
71.6
-
acetyl-CoA at pH 8.0 and 20°C Arabidopsis thaliana
90.3
-
oxaloacetate at pH 8.0 and 20°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate Arabidopsis thaliana
-
citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P20115
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Arabidopsis thaliana citrate + CoA
-
?

Subunits

Subunits Comment Organism
dimer or oligomer the CS4 isoform is not only present as dimer but also as high-molecular-weight oligomer under native conditions Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CS4 isoform Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35
-
nearly 50% of the activity is retained at 35°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5 9 nearly 50% of the activity is retained at pH 5.0 or 9.0 Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1827
-
oxaloacetate at pH 8.0 and 20°C Arabidopsis thaliana
4339
-
acetyl-CoA at pH 8.0 and 20°C Arabidopsis thaliana