Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | a strong and more than 8fold activation of enzyme activity is observed for isoform CS4 with Escherichia coli thioredoxin coupled to reduction by NTR and NADPH | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | about 54% inhibition at 0.4 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
71.6 | - |
acetyl-CoA | at pH 8.0 and 20°C | Arabidopsis thaliana | |
90.3 | - |
oxaloacetate | at pH 8.0 and 20°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | Arabidopsis thaliana | - |
citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P20115 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Arabidopsis thaliana | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer or oligomer | the CS4 isoform is not only present as dimer but also as high-molecular-weight oligomer under native conditions | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
CS4 | isoform | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
nearly 50% of the activity is retained at 35°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | nearly 50% of the activity is retained at pH 5.0 or 9.0 | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1827 | - |
oxaloacetate | at pH 8.0 and 20°C | Arabidopsis thaliana | |
4339 | - |
acetyl-CoA | at pH 8.0 and 20°C | Arabidopsis thaliana |