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Literature summary for 2.3.3.16 extracted from
- Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula (2019), PLoS ONE, 14, e0212807 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure at 1.7 A resolution. Like other Type-I CS, citrate synthase functions as a dimer and each monomer consists of a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site is more closed upon binding of the oxaloacetate substrate than binding of the citrate product | Metallosphaera sedula |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | noncompetitive | Metallosphaera sedula |  |
| citrate | product inhibition, competitive. Citrate binds tightly to the substrate binding site and its binding induces a compact closed conformation | Metallosphaera sedula | |
| NADH | noncompetitive | Metallosphaera sedula | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0165 | - |
acetyl-CoA | pH 8.0, 25°C | Metallosphaera sedula | |
| 0.0414 | - |
oxaloacetate | pH 8.0, 25°C | Metallosphaera sedula | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metallosphaera sedula | A4YGX6 | - |
- |
| Metallosphaera sedula DSM 5348 | A4YGX6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | - |
Metallosphaera sedula | citrate + CoA | - |
? | |
| acetyl-CoA + H2O + oxaloacetate | - |
Metallosphaera sedula DSM 5348 | citrate + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Msed_1522 | - |
Metallosphaera sedula |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.62 | - |
oxaloacetate | pH 8.0, 25°C | Metallosphaera sedula | |
| 8.56 | - |
acetyl-CoA | pH 8.0, 25°C | Metallosphaera sedula | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.34 | - |
ATP | substrate oxaloacetate, pH 8.0, 25°C | Metallosphaera sedula | |
| 4.41 | - |
NADH | substrate oxaloacetate, pH 8.0, 25°C | Metallosphaera sedula | |
| 7.19 | - |
citrate | substrate oxaloacetate, pH 8.0, 25°C | Metallosphaera sedula | |
| 7.96 | - |
NADH | substrate acetyl-CoA, pH 8.0, 25°C | Metallosphaera sedula | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 184 | - |
oxaloacetate | pH 8.0, 25°C | Metallosphaera sedula | |
| 519 | - |
acetyl-CoA | pH 8.0, 25°C | Metallosphaera sedula | |
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Release 2023.1 (January 2023)
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