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Literature summary for 2.3.3.16 extracted from

  • Ito, S.; Koyama, N.; Osanai, T.
    Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase (2019), Sci. Rep., 9, 6038 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.16

Activating Compound

Activating Compound Comment Organism Structure
CaCl2 100 mM, 151% increase in activity Synechocystis sp. PCC 6803
KCl 100 mM, 88% increase in activity. Activation is pH dependent Synechocystis sp. PCC 6803
MgCl2 100 mM, 291% increase in activity Synechocystis sp. PCC 6803
NaCl 100 mM, 105% increase in activity Synechocystis sp. PCC 6803

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechocystis sp. PCC 6803

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 5 mM, 2% residual activity Synechocystis sp. PCC 6803
citrate 5 mM, 1% residual activity Synechocystis sp. PCC 6803
Mn2+ 100 mM, 48% residual activity Synechocystis sp. PCC 6803
phosphoenolpyruvate 5 mM, 14% residual activity Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
 oxaloacetate presence of 5 mM ADP, pH 7.5, 37°C Synechocystis sp. PCC 6803
0.091
-
 oxaloacetate pH 7.5, 37°C Synechocystis sp. PCC 6803
0.108
-
 oxaloacetate presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C Synechocystis sp. PCC 6803
0.153
-
 acetyl-CoA presence of 5 mM ADP, pH 7.5, 37°C Synechocystis sp. PCC 6803
0.194
-
 acetyl-CoA presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C Synechocystis sp. PCC 6803
0.22
-
 acetyl-CoA pH 7.5, 37°C Synechocystis sp. PCC 6803

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 Q59977
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
 Synechocystis sp. PCC 6803 citrate + CoA
-
 ir

Synonyms

Synonyms Comment Organism
sll0401
-
 Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Synechocystis sp. PCC 6803

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.42
-
 acetyl-CoA presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C Synechocystis sp. PCC 6803
1.53
-
 oxaloacetate presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C Synechocystis sp. PCC 6803
2.51
-
 acetyl-CoA pH 7.5, 37°C Synechocystis sp. PCC 6803
2.76
-
 oxaloacetate pH 7.5, 37°C Synechocystis sp. PCC 6803
2.99
-
 acetyl-CoA presence of 5 mM ADP, pH 7.5, 37°C Synechocystis sp. PCC 6803
3.35
-
 oxaloacetate presence of 5 mM ADP, pH 7.5, 37°C Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Synechocystis sp. PCC 6803

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.4
-
 acetyl-CoA presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C Synechocystis sp. PCC 6803
12.1
-
 acetyl-CoA pH 7.5, 37°C Synechocystis sp. PCC 6803
14.2
-
 oxaloacetate presence of 5 mM phosphoenolpyruvate, pH 7.5, 37°C Synechocystis sp. PCC 6803
19.9
-
 acetyl-CoA presence of 5 mM ADP, pH 7.5, 37°C Synechocystis sp. PCC 6803
30.5
-
 oxaloacetate pH 7.5, 37°C Synechocystis sp. PCC 6803
45.65
-
 oxaloacetate presence of 5 mM ADP, pH 7.5, 37°C Synechocystis sp. PCC 6803